Veli2 CRISPR/Cas9 KO Plasmid (m): sc-423668
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Veli2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Veli2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Veli2 CRISPR/Cas9 KO Plasmid (m) | sc-423668 | 20 µg | $397.00 |
Overview
Lin7b encodes Veli2, a membrane-associated scaffolding protein in the MAGUK/LIN7 family that helps organize basolateral polarity complexes and stabilize membrane protein trafficking. Veli2 participates in assembly of junctional and synaptic protein networks through interactions with PDZ domain–containing partners, influencing epithelial polarity, neuronal signaling, and vesicular transport. In mouse systems, disruption of LIN7-associated complexes can perturb cell–cell adhesion, receptor localization, and excitatory synapse organization, processes frequently interrogated in neurodevelopmental and barrier-function models. Altered polarity and trafficking pathways are widely relevant to mechanisms underlying developmental phenotypes and disease-associated dysregulation of tissue architecture and neuronal connectivity.
Veli2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Lin7b gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Lin7b together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Lin7b open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Veli2 protein expression.
This CRISPR knockout system enables efficient generation of Lin7b-deficient cell models for investigation of Veli2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Lin7b exon(s) critical for Veli2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Lin7b genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Veli2 CRISPR/Cas9 KO Plasmid (m) and Veli2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Lin7b locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Veli2 HDR Plasmid (m) and Veli2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Lin7b homology arms to support homology-directed repair at defined Lin7b target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.