Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
VDAC2 CRISPR/Cas9 KO Plasmid (m): sc-423662
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- VDAC2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the VDAC2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VDAC2 CRISPR/Cas9 KO Plasmid (m) | sc-423662 | 20 µg | $397.00 |
Overview
Vdac2 encodes voltage-dependent anion channel 2 (VDAC2), a major porin of the outer mitochondrial membrane that regulates metabolite and ion flux between the cytosol and mitochondria. VDAC2 participates in mitochondrial respiration, redox homeostasis, and Ca²⁺-dependent signaling by influencing ATP/ADP exchange and coupling to inner membrane transport processes. It also modulates intrinsic apoptosis by controlling mitochondrial outer membrane permeabilization and interactions with BCL-2 family proteins, linking mitochondrial metabolism to cell fate decisions. Dysregulation of VDAC2-associated pathways has been implicated in altered bioenergetics and stress responses relevant to neurodegeneration, cardiometabolic disease mechanisms, and cancer cell survival phenotypes.
VDAC2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Vdac2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Vdac2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Vdac2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish VDAC2 protein expression.
This CRISPR knockout system enables efficient generation of Vdac2-deficient cell models for investigation of VDAC2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Vdac2 exon(s) critical for VDAC2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Vdac2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by VDAC2 CRISPR/Cas9 KO Plasmid (m) and VDAC2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Vdac2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by VDAC2 HDR Plasmid (m) and VDAC2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Vdac2 homology arms to support homology-directed repair at defined Vdac2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.