Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
VDAC1/Porin Double Nickase Plasmid (h): sc-418200-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- VDAC1/Porin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- VDAC1/Porin Double Nickase Plasmid (h) and VDAC1/Porin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting VDAC1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: VDAC1/Porin Antibody (B-6): sc-390996
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VDAC1/Porin Double Nickase Plasmid (h) | sc-418200-NIC | 20 µg | $410.00 | |||
VDAC1/Porin Double Nickase Plasmid (h2) | sc-418200-NIC-2 | 20 µg | $410.00 |
VDAC1 (voltage-dependent anion channel 1), also known as porin, is a principal conduit in the mitochondrial outer membrane that mediates exchange of ATP/ADP, ions, and key metabolites between mitochondria and cytosol. By controlling outer membrane permeability and coordinating interactions with hexokinase and BCL-2 family proteins, VDAC1 integrates cellular energy metabolism with apoptotic signaling, calcium homeostasis, and redox balance. VDAC1 function intersects with oxidative phosphorylation, glycolytic coupling, and mitochondria-associated membrane processes that shape mitochondrial dynamics and stress responses. Dysregulated VDAC1 expression or channel activity has been linked to altered bioenergetics and mitochondrial dysfunction observed in cancer biology, neurodegeneration, and metabolic disease research.
VDAC1/Porin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the VDAC1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within VDAC1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt VDAC1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of VDAC1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.