Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
UTY CRISPR Activation Plasmid (h): sc-416327-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UTY CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- UTY CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by UTY CRISPR Activation Plasmid (h) and UTY CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the UTY transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: UTY Antibody (G-3): sc-514690
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UTY CRISPR Activation Plasmid (h) | sc-416327-ACT | 20 µg | $397.00 |
UTY (ubiquitously transcribed tetratricopeptide repeat gene, Y-linked) encodes a nuclear chromatin-associated protein in the KDM6 family that participates in epigenetic control of transcriptional programs. Although its catalytic demethylase activity is reduced relative to paralogs, UTY is implicated in regulation of histone H3K27 methylation states, chromatin remodeling, and developmental gene expression networks. UTY-dependent chromatin processes intersect with lineage specification, immune cell differentiation, and maintenance of transcriptional states in proliferating cells. Altered UTY dosage or Y-chromosome–linked regulation has been investigated in the context of sex-biased molecular phenotypes and disease-relevant transcriptional dysregulation, including cancer-associated epigenomic changes.
UTY CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous UTY expression without altering the underlying DNA sequence.
UTY CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the UTY locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the UTY transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous UTY expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native UTY locus and enabling the study of UTY-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of UTY pathway restoration in tumor cells with silenced or reduced UTY expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.