Uroguanylin Double Nickase Plasmid (h): sc-404519-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Uroguanylin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Uroguanylin Double Nickase Plasmid (h) and Uroguanylin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GUCA2B. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Uroguanylin Double Nickase Plasmid (h) | sc-404519-NIC | 20 µg | $410.00 | |||
Uroguanylin Double Nickase Plasmid (h2) | sc-404519-NIC-2 | 20 µg | $410.00 |
GUCA2B encodes uroguanylin, a secreted peptide hormone that activates the guanylate cyclase C (GUCY2C) receptor on intestinal epithelial cells to elevate intracellular cGMP. This signaling axis regulates electrolyte and water transport, epithelial barrier function, and mucosal homeostasis through cGMP-dependent pathways that influence ion channel activity and downstream kinases. Uroguanylin is integral to gut–kidney axis communication and contributes to renal natriuresis and blood pressure-related physiology via cGMP signaling. Dysregulation of GUCA2B/GUCY2C-cGMP signaling has been linked to altered intestinal secretion and barrier integrity, with implications for inflammatory conditions and epithelial pathobiology relevant to colorectal disease research.
Uroguanylin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GUCA2B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GUCA2B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GUCA2B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GUCA2B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.