Ufc1 CRISPR/Cas9 KO Plasmid (h): sc-409943
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ufc1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Ufc1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ufc1 CRISPR/Cas9 KO Plasmid (h) | sc-409943 | 20 µg | $397.00 |
Overview
UFC1 encodes the E2-like conjugating enzyme Ufc1, a core component of the UFM1 (ubiquitin-fold modifier 1) conjugation system that drives ufmylation of protein substrates. This pathway coordinates proteostasis at the endoplasmic reticulum, ER stress signaling, and quality control processes linked to secretory pathway function, with reported roles in ribosome-associated quality control and cellular homeostasis. Disruption of ufmylation components, including UFC1, has been associated with neurodevelopmental phenotypes, hematopoietic defects, and cancer-related cellular stress adaptation in experimental models, supporting its relevance to disease biology. UFC1 is therefore frequently studied in contexts where ER function, stress resilience, and proteome maintenance influence cell fate decisions.
Ufc1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the UFC1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the UFC1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the UFC1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Ufc1 protein expression.
This CRISPR knockout system enables efficient generation of UFC1-deficient cell models for investigation of Ufc1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting UFC1 exon(s) critical for Ufc1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple UFC1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Ufc1 CRISPR/Cas9 KO Plasmid (h) and Ufc1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the UFC1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Ufc1 HDR Plasmid (h) and Ufc1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by UFC1 homology arms to support homology-directed repair at defined UFC1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.