UBA52 CRISPR/Cas9 KO Plasmid (m): sc-423573
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UBA52 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the UBA52 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UBA52 CRISPR/Cas9 KO Plasmid (m) | sc-423573 | 20 µg | $397.00 |
Overview
Uba52 encodes a ubiquitin–ribosomal protein fusion (UBA52) that is processed to yield ubiquitin and ribosomal protein L40, linking ubiquitin availability to ribosome biogenesis and global proteostasis. Through ubiquitin-dependent pathways, UBA52 supports regulated protein turnover via the proteasome, influences cellular stress responses, and contributes to control of translation. Perturbations in ubiquitin homeostasis and ribosomal function are broadly relevant to mechanisms of proteotoxic stress, cell cycle progression, and inflammatory signaling. Because ubiquitination and translational control are frequently altered in cancer biology and neurodegeneration models, Uba52 serves as a useful node for studying how proteostasis impacts cell fitness and stress adaptation.
UBA52 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Uba52 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Uba52 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Uba52 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish UBA52 protein expression.
This CRISPR knockout system enables efficient generation of Uba52-deficient cell models for investigation of UBA52 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Uba52 exon(s) critical for UBA52 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Uba52 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by UBA52 CRISPR/Cas9 KO Plasmid (m) and UBA52 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Uba52 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by UBA52 HDR Plasmid (m) and UBA52 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Uba52 homology arms to support homology-directed repair at defined Uba52 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.