TRIM8 Double Nickase Plasmid (m): sc-429995-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TRIM8 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TRIM8 Double Nickase Plasmid (m) and TRIM8 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Trim8. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TRIM8 Antibody (B-3): sc-398878
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRIM8 Double Nickase Plasmid (m) | sc-429995-NIC | 20 µg | $410.00 | |||
TRIM8 Double Nickase Plasmid (m2) | sc-429995-NIC-2 | 20 µg | $410.00 |
Trim8 encodes TRIM8, a RING-type E3 ubiquitin ligase in the TRIM family that regulates protein stability and signaling through ubiquitin-dependent mechanisms. In mouse cells, TRIM8 has been linked to modulation of key stress and inflammatory pathways, including p53-associated responses, NF-κB signaling, and JAK/STAT pathway outputs, influencing proliferation, apoptosis, and cytokine-driven transcriptional programs. Through these roles, TRIM8 is frequently studied in contexts of genome integrity, innate immune regulation, and cell fate decisions. Dysregulated TRIM8 activity has been associated with pathological processes involving aberrant inflammatory signaling and altered tumor suppressor network function, supporting its relevance in disease-model research.
TRIM8 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Trim8 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Trim8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Trim8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Trim8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.