Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TFEB Double Nickase Plasmid (h): sc-401388-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TFEB Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TFEB Double Nickase Plasmid (h) and TFEB Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TFEB. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TFEB Antibody (C-6): sc-166736
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TFEB Double Nickase Plasmid (h) | sc-401388-NIC | 20 µg | $410.00 | |||
TFEB Double Nickase Plasmid (h2) | sc-401388-NIC-2 | 20 µg | $410.00 |
TFEB encodes transcription factor EB, a master regulator of lysosomal biogenesis and autophagy that coordinates expression of the CLEAR gene network. By integrating signals from mTORC1 and nutrient sensing pathways, TFEB controls lysosome function, autophagosome–lysosome fusion, and cellular adaptation to stress, including oxidative and proteotoxic stress. Dysregulated TFEB activity has been linked to altered proteostasis and organelle quality control in neurodegeneration, lysosomal storage disorders, metabolic disease, and cancer biology, making it a central node for studies of intracellular clearance mechanisms. In human cells, TFEB-dependent transcriptional programs influence immune signaling and inflammatory remodeling through effects on endolysosomal trafficking and antigen processing.
TFEB Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TFEB locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TFEB. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TFEB function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TFEB-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.