Telethonin CRISPR/Cas9 KO Plasmid (h): sc-402317
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Telethonin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Telethonin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Telethonin Antibody (G-11): sc-25327
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Telethonin CRISPR/Cas9 KO Plasmid (h) | sc-402317 | 20 µg | $397.00 |
Overview
TCAP encodes telethonin, a Z-disc protein that binds titin (TTN) and contributes to sarcomere assembly, mechanosensing, and structural stability of striated muscle. Telethonin participates in tension-dependent signaling networks linking the contractile apparatus to protein quality control pathways, including ubiquitin–proteasome-mediated turnover and stress-responsive remodeling. Disruption of TCAP perturbs myofibrillar integrity and has been associated with inherited cardiomyopathies and muscular dystrophy phenotypes in genetic and functional studies. As a muscle-restricted scaffold, telethonin is widely studied in cardiomyocyte differentiation, sarcomere maintenance, and biomechanical stress adaptation.
Telethonin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TCAP gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TCAP together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TCAP open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Telethonin protein expression.
This CRISPR knockout system enables efficient generation of TCAP-deficient cell models for investigation of Telethonin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TCAP exon(s) critical for Telethonin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TCAP genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Telethonin CRISPR/Cas9 KO Plasmid (h) and Telethonin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TCAP locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Telethonin HDR Plasmid (h) and Telethonin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TCAP homology arms to support homology-directed repair at defined TCAP target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.