TEF-5 CRISPR/Cas9 KO Plasmid (m): sc-423328
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TEF-5 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TEF-5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TEF-5 CRISPR/Cas9 KO Plasmid (m) | sc-423328 | 20 µg | $397.00 |
Overview
Tead3 encodes TEF-5, a TEA/ATTS domain transcription factor that partners with co-activators such as YAP/TAZ to regulate enhancer-driven gene expression programs controlling cell proliferation, survival, and lineage specification. In mouse, TEF-5 contributes to developmental patterning and organ growth by integrating Hippo pathway signals with context-dependent transcriptional outputs. TEAD family activity is widely linked to mechanotransduction and stem/progenitor cell states, making Tead3 a relevant node for studying growth control and tissue homeostasis. Dysregulated TEAD–YAP/TAZ transcriptional circuitry is associated with tumorigenic phenotypes and aberrant regeneration in multiple model systems, supporting its use in pathway and disease-mechanism research.
TEF-5 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tead3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tead3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tead3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TEF-5 protein expression.
This CRISPR knockout system enables efficient generation of Tead3-deficient cell models for investigation of TEF-5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tead3 exon(s) critical for TEF-5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tead3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TEF-5 CRISPR/Cas9 KO Plasmid (m) and TEF-5 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tead3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TEF-5 HDR Plasmid (m) and TEF-5 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tead3 homology arms to support homology-directed repair at defined Tead3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.