Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TEF-3 CRISPR/Cas9 KO Plasmid (m2): sc-423329-KO-2
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TEF-3 CRISPR/Cas9 Knockout (KO) Plasmid (m2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TEF-3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TEF-3 Antibody (N-G2): sc-101184
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TEF-3 CRISPR/Cas9 KO Plasmid (m2) | sc-423329-KO-2 | 20 µg | $397.00 |
Overview
Tead4 encodes the transcription factor TEF-3, a TEA/ATTS domain DNA-binding protein that partners with co-activators such as YAP/TAZ to regulate gene programs controlling proliferation, survival, and cell fate decisions. In mouse development, TEAD4 is a key determinant of trophectoderm specification and contributes to epithelial organization and lineage commitment through Hippo pathway–responsive transcription. TEF-3 activity integrates mechanical cues and upstream kinase signaling to modulate target genes involved in growth control and differentiation. Dysregulated TEAD-family signaling has been linked to altered tissue homeostasis and oncogenic transcriptional programs, making Tead4 a useful node for studying context-dependent transcriptional regulation.
TEF-3 CRISPR/Cas9 KO Plasmid (m2) is a pool of plasmids designed for targeted disruption of the Tead4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tead4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tead4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TEF-3 protein expression.
This CRISPR knockout system enables efficient generation of Tead4-deficient cell models for investigation of TEF-3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tead4 exon(s) critical for TEF-3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tead4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TEF-3 CRISPR/Cas9 KO Plasmid (m) and TEF-3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tead4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TEF-3 HDR Plasmid (m) and TEF-3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tead4 homology arms to support homology-directed repair at defined Tead4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.