TdTIF1 Antibody (G-2): sc-166296
- TdTIF1 Antibody (G-2) is a mouse monoclonal IgG2b κ TdTIF1 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 228-297 mapping near the C-terminus of TdTIF1 of human origin
- TdTIF1 Antibody (G-2) is recommended for detection of TdTIF1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-TdTIF1 Antibody (G-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TdTIF1 Antibody (G-2) for WB and IHC(P) applications. These reagents are now offered in bundles with TdTIF1 Antibody (G-2) (see ordering information below).
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SEE ALSO...
TdTIF1 Antibody (G-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects TdTIF1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). TdTIF1 (G-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Terminal deoxynucleotidyltransferase (TdT) is a crucial DNA polymerase that catalyzes the addition of deoxyribonucleotides to the 3′-hydroxyl end of DNA primers without requiring a DNA template, which is essential for processes such as DNA repair and replication. The regulation of TdT activity is vital, as specific TdT-interacting factors can positively or negatively influence TdT activity. TdTIF1, also known as DNTTIP1 or TdIF1, is localized in the nucleus and plays a significant role in repressing TdT activity by binding to the Pol β-like region of TdT, effectively masking its DNA-binding site and preventing DNA interaction. TdTIF1 is expressed throughout the body, with the highest levels found in the thymus, and features N-terminal AT-hook motifs that facilitate TdT binding. Beyond its inhibitory role, TdTIF1 also interacts with TReP-132, a transcriptional co-activator of steroidogenic factor 1 (SF-1), enhancing gene expression in steroid-producing cells when bound to SF-1.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TdTIF1 Antibody (G-2) References:
- Terminal deoxynucleotidyltransferase directly interacts with a novel nuclear protein that is homologous to p65. | Yamashita, N., et al. 2001. Genes Cells. 6: 641-52. PMID: 11473582
- Terminal deoxynucleotidyltransferase forms a ternary complex with a novel chromatin remodeling protein with 82 kDa and core histone. | Fujita, K., et al. 2003. Genes Cells. 8: 559-71. PMID: 12786946
- Direct binding of TReP-132 with TdT results in reduction of TdT activity. | Fujisaki, S., et al. 2006. Genes Cells. 11: 47-57. PMID: 16371131
- Identification of functional domains in TdIF1 and its inhibitory mechanism for TdT activity. | Kubota, T., et al. 2007. Genes Cells. 12: 941-59. PMID: 17663723
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TdTIF1 Antibody (G-2) | sc-166296 | 200 µg/ml | $322.00 | |||
TdTIF1 Antibody (G-2): m-IgGκ BP-HRP Bundle | sc-521559 | 200 µg Ab, 40 µg BP | $361.00 | |||
TdTIF1 Antibody (G-2): m-IgG2b BP-HRP Bundle | sc-548927 | 200 µg Ab; 10 µg BP | $361.00 | |||
TdTIF1 Antibody (G-2) AC | sc-166296 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TdTIF1 Antibody (G-2) HRP | sc-166296 HRP | 200 µg/ml | $322.00 | |||
TdTIF1 Antibody (G-2) FITC | sc-166296 FITC | 200 µg/ml | $336.00 | |||
TdTIF1 Antibody (G-2) PE | sc-166296 PE | 200 µg/ml | $349.00 | |||
TdTIF1 Antibody (G-2) Alexa Fluor® 488 | sc-166296 AF488 | 200 µg/ml | $364.00 | |||
TdTIF1 Antibody (G-2) Alexa Fluor® 546 | sc-166296 AF546 | 200 µg/ml | $364.00 | |||
TdTIF1 Antibody (G-2) Alexa Fluor® 594 | sc-166296 AF594 | 200 µg/ml | $364.00 | |||
TdTIF1 Antibody (G-2) Alexa Fluor® 647 | sc-166296 AF647 | 200 µg/ml | $364.00 | |||
TdTIF1 Antibody (G-2) Alexa Fluor® 680 | sc-166296 AF680 | 200 µg/ml | $364.00 | |||
TdTIF1 Antibody (G-2) Alexa Fluor® 790 | sc-166296 AF790 | 200 µg/ml | $364.00 |