TAL2 CRISPR/Cas9 KO Plasmid (h): sc-406836
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TAL2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TAL2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TAL2 Antibody (1G6): sc-517121
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TAL2 CRISPR/Cas9 KO Plasmid (h) | sc-406836 | 20 µg | $397.00 |
Overview
TAL2 (T-cell acute lymphocytic leukemia 2) encodes a basic helix–loop–helix transcription factor that contributes to lineage specification and regulation of gene expression programs during hematopoietic development. TAL2 can participate in transcriptional complexes that influence chromatin accessibility and coordinate differentiation versus proliferation decisions, intersecting with broader bHLH-driven regulatory networks. Dysregulated TAL2 expression has been linked to aberrant transcriptional control in T-lineage contexts and is studied in models of leukemogenesis and altered immune cell development. As a nuclear regulatory protein, TAL2 provides a tractable entry point for dissecting transcriptional circuitry, cell fate transitions, and oncogenic transcription factor dependencies.
TAL2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TAL2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TAL2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TAL2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TAL2 protein expression.
This CRISPR knockout system enables efficient generation of TAL2-deficient cell models for investigation of TAL2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TAL2 exon(s) critical for TAL2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TAL2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TAL2 CRISPR/Cas9 KO Plasmid (h) and TAL2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TAL2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TAL2 HDR Plasmid (h) and TAL2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TAL2 homology arms to support homology-directed repair at defined TAL2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.