Syntaxin 10 CRISPR/Cas9 KO Plasmid (h): sc-406240
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Syntaxin 10 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Syntaxin 10 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Syntaxin 10 CRISPR/Cas9 KO Plasmid (h) | sc-406240 | 20 µg | $397.00 |
Overview
STX10 encodes Syntaxin 10, a t-SNARE protein that localizes primarily to the trans-Golgi network and endosomal membranes where it regulates vesicle docking and membrane fusion. Through interactions with other SNARE components and tethering factors, Syntaxin 10 helps control endosome-to-Golgi retrieval and post-Golgi trafficking, supporting receptor recycling, lysosome-related transport, and overall secretory pathway homeostasis. Disruption of STX10-dependent trafficking can alter cargo sorting and organelle dynamics, processes frequently implicated in immune signaling, neurobiology, and cellular stress responses. Altered vesicular transport programs involving SNARE machinery have been associated with disease-relevant phenotypes including dysregulated inflammation and neurodevelopmental dysfunction in model systems.
Syntaxin 10 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the STX10 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the STX10 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the STX10 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Syntaxin 10 protein expression.
This CRISPR knockout system enables efficient generation of STX10-deficient cell models for investigation of Syntaxin 10 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting STX10 exon(s) critical for Syntaxin 10 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple STX10 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Syntaxin 10 CRISPR/Cas9 KO Plasmid (h) and Syntaxin 10 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the STX10 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Syntaxin 10 HDR Plasmid (h) and Syntaxin 10 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by STX10 homology arms to support homology-directed repair at defined STX10 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.