Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Synaptoporin CRISPR/Cas9 KO Plasmid (m): sc-428189
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Synaptoporin CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Synaptoporin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Synaptoporin Antibody (C-9): sc-376761
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Synaptoporin CRISPR/Cas9 KO Plasmid (m) | sc-428189 | 20 µg | $397.00 |
Overview
Synpr encodes synaptoporin, a synaptic vesicle membrane protein enriched in presynaptic terminals of mouse neurons and related to the synaptophysin family. Synaptoporin contributes to synaptic vesicle biogenesis and recycling, influencing neurotransmitter release and short-term synaptic plasticity through activity-dependent trafficking and endocytosis/exocytosis coupling. It is frequently studied in the context of hippocampal and glutamatergic circuitry where vesicle dynamics shape network excitability. Altered presynaptic vesicle protein composition, including synaptoporin-associated pathways, is relevant to investigations of neurodevelopmental and neurodegenerative disease mechanisms without implying clinical outcomes.
Synaptoporin CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Synpr gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Synpr together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Synpr open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Synaptoporin protein expression.
This CRISPR knockout system enables efficient generation of Synpr-deficient cell models for investigation of Synaptoporin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Synpr exon(s) critical for Synaptoporin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Synpr genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Synaptoporin CRISPR/Cas9 KO Plasmid (m) and Synaptoporin CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Synpr locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Synaptoporin HDR Plasmid (m) and Synaptoporin HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Synpr homology arms to support homology-directed repair at defined Synpr target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.