Synapsin IIa CRISPR/Cas9 KO Plasmid (m): sc-423234
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Synapsin IIa CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Synapsin IIa genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Synapsin IIa Antibody (1): sc-136086
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Synapsin IIa CRISPR/Cas9 KO Plasmid (m) | sc-423234 | 20 µg | $397.00 |
Overview
Syn2 encodes synapsin IIa, a presynaptic phosphoprotein that associates with synaptic vesicles and actin to regulate vesicle clustering, reserve pool maintenance, and activity-dependent neurotransmitter release. Synapsin IIa is controlled by phosphorylation downstream of neuronal signaling cascades including PKA, CaMKII, and MAPK/ERK, linking calcium influx and second-messenger pathways to synaptic plasticity. In mouse nervous system models, altered synapsin function is used to study changes in excitation–inhibition balance, network synchronization, and synaptic maturation that are relevant to neurodevelopmental and neuropsychiatric phenotypes. Disruption of Syn2 provides a tractable approach to interrogate presynaptic mechanisms influencing circuit stability and stimulus-evoked synaptic transmission.
Synapsin IIa CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Syn2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Syn2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Syn2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Synapsin IIa protein expression.
This CRISPR knockout system enables efficient generation of Syn2-deficient cell models for investigation of Synapsin IIa signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Syn2 exon(s) critical for Synapsin IIa function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Syn2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Synapsin IIa CRISPR/Cas9 KO Plasmid (m) and Synapsin IIa CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Syn2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Synapsin IIa HDR Plasmid (m) and Synapsin IIa HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Syn2 homology arms to support homology-directed repair at defined Syn2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.