Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SLUG Double Nickase Plasmid (h): sc-400389-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SLUG Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SLUG Double Nickase Plasmid (h) and SLUG Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SNAI2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SLUG Antibody (A-7): sc-166476
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SLUG Double Nickase Plasmid (h) | sc-400389-NIC | 20 µg | $410.00 | |||
SLUG Double Nickase Plasmid (h2) | sc-400389-NIC-2 | 20 µg | $410.00 |
SNAI2 encodes the zinc-finger transcription factor SLUG, a key regulator of epithelial–mesenchymal transition (EMT) that represses epithelial genes such as CDH1 and remodels cytoskeletal and adhesion programs to promote motility and cell-state plasticity. SLUG integrates signals from developmental and stress-response pathways, including TGF-β, WNT/β-catenin, Notch, and MAPK, to coordinate lineage decisions, survival, and differentiation. In human biology, altered SNAI2 activity is frequently linked to tumor progression phenotypes, invasion-associated transcriptional programs, and therapy-adaptive states, and it also contributes to neural crest and hematopoietic contexts. These properties make SLUG a widely used node for dissecting transcriptional control of EMT, cell migration, stem-like traits, and microenvironmental responses.
SLUG Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SNAI2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SNAI2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SNAI2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SNAI2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.