Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Six1 CRISPR/Cas9 KO Plasmid (m): sc-422956
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Six1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Six1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Six1 Antibody (B-8): sc-514441
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Six1 CRISPR/Cas9 KO Plasmid (m) | sc-422956 | 20 µg | $397.00 |
Overview
Six1 (sine oculis homeobox homolog 1) encodes a homeobox transcription factor that regulates embryonic patterning and organogenesis in mouse, with prominent roles in myogenesis, nephrogenesis, and sensory placode development. SIX1 coordinates lineage specification and proliferation programs through transcriptional control networks that interface with developmental signaling pathways, including interactions with EYA co-activators and modulation of growth and differentiation gene sets. Dysregulated SIX1 expression or activity is associated with aberrant developmental trajectories and has been studied in the context of congenital anomalies and oncogenic transcriptional programs. In cells, Six1-dependent transcriptional circuits influence cell-cycle progression, migration, and epithelial–mesenchymal state transitions relevant to developmental biology and disease modeling.
Six1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Six1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Six1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Six1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Six1 protein expression.
This CRISPR knockout system enables efficient generation of Six1-deficient cell models for investigation of Six1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Six1 exon(s) critical for Six1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Six1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Six1 CRISPR/Cas9 KO Plasmid (m) and Six1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Six1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Six1 HDR Plasmid (m) and Six1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Six1 homology arms to support homology-directed repair at defined Six1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.