Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SIRT1 Double Nickase Plasmid (m): sc-430046-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SIRT1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SIRT1 Double Nickase Plasmid (m) and SIRT1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Sirt1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SIRT1 Antibody (B-7): sc-74465
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SIRT1 Double Nickase Plasmid (m) | sc-430046-NIC | 20 µg | $410.00 | |||
SIRT1 Double Nickase Plasmid (m2) | sc-430046-NIC-2 | 20 µg | $410.00 |
Mouse Sirt1 encodes SIRT1, a NAD⁺-dependent deacetylase that couples cellular energy status to chromatin remodeling and transcriptional control. SIRT1 regulates acetylation of key factors including p53, FOXO proteins, PGC-1α, and NF-κB, thereby influencing stress responses, mitochondrial biogenesis, inflammation, autophagy, and cell cycle checkpoints. Through crosstalk with AMPK–mTOR signaling and metabolic pathways, SIRT1 contributes to adaptive responses to nutrient availability and oxidative stress. Altered SIRT1 activity has been associated with metabolic dysfunction, neurodegeneration-related processes, and tumor biology in model systems, making it a widely studied node in epigenetic and signaling networks.
SIRT1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Sirt1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Sirt1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Sirt1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Sirt1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.