Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SIRP-α CRISPR/Cas9 KO Plasmid (h): sc-401201
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SIRP-α CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SIRP-α genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SIRP-α Antibody (C-7): sc-376884
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SIRP-α CRISPR/Cas9 KO Plasmid (h) | sc-401201 | 20 µg | $397.00 |
Overview
Signal regulatory protein alpha (SIRP-α), encoded by human SIRPA, is an immunoglobulin superfamily transmembrane receptor prominently expressed on myeloid cells where it modulates innate immune activation. Through phosphorylation of cytoplasmic ITIM motifs and recruitment of SHP-1/SHP-2 phosphatases, SIRP-α transduces inhibitory signaling downstream of CD47 engagement to regulate cytoskeletal dynamics, phagocytosis, adhesion, and cytokine responses. This CD47–SIRP-α axis shapes macrophage and dendritic cell behavior within inflammatory microenvironments and has been implicated in tumor immune evasion, chronic inflammation, and dysregulated myeloid function. SIRPA is therefore widely studied in pathways controlling immune homeostasis, antigen-presenting cell function, and cell–cell recognition.
SIRP-α CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SIRPA gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SIRPA together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SIRPA open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SIRP-α protein expression.
This CRISPR knockout system enables efficient generation of SIRPA-deficient cell models for investigation of SIRP-α signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SIRPA exon(s) critical for SIRP-α function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SIRPA genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SIRP-α CRISPR/Cas9 KO Plasmid (h) and SIRP-α CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SIRPA locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SIRP-α HDR Plasmid (h) and SIRP-α HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SIRPA homology arms to support homology-directed repair at defined SIRPA target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.