SDS3 CRISPR/Cas9 KO Plasmid (h): sc-413010
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SDS3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SDS3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SDS3 CRISPR/Cas9 KO Plasmid (h) | sc-413010 | 20 µg | $397.00 |
Overview
SUDS3 encodes SDS3, a core component of the Sin3A/HDAC transcriptional corepressor complex that couples chromatin remodeling to histone deacetylation. Through recruitment of HDAC1/2 and associated factors, SDS3 contributes to transcriptional repression programs that shape cell-cycle progression, lineage specification, and responses to developmental and stress cues. SDS3-dependent chromatin regulation influences genome stability and epigenetic homeostasis, linking altered complex composition or activity to aberrant gene expression states. Dysregulation of Sin3/HDAC-mediated repression and related epigenetic pathways is frequently implicated in cancer biology and in broader contexts of neurodevelopmental and differentiation-associated phenotypes.
SDS3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SUDS3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SUDS3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SUDS3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SDS3 protein expression.
This CRISPR knockout system enables efficient generation of SUDS3-deficient cell models for investigation of SDS3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SUDS3 exon(s) critical for SDS3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SUDS3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SDS3 CRISPR/Cas9 KO Plasmid (h) and SDS3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SUDS3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SDS3 HDR Plasmid (h) and SDS3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SUDS3 homology arms to support homology-directed repair at defined SUDS3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.