Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SDHA Double Nickase Plasmid (m): sc-426283-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SDHA Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SDHA Double Nickase Plasmid (m) and SDHA Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Sdha. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SDHA Antibody (F-2): sc-390381
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SDHA Double Nickase Plasmid (m) | sc-426283-NIC | 20 µg | $410.00 |
Mouse Sdha encodes the flavoprotein subunit of succinate dehydrogenase (SDHA), a core component of mitochondrial complex II that links the tricarboxylic acid cycle to the electron transport chain by catalyzing succinate-to-fumarate oxidation and funneling electrons to ubiquinone. Through this dual role, SDHA helps regulate oxidative phosphorylation, mitochondrial redox balance, and cellular bioenergetics, with downstream effects on reactive oxygen species and metabolite signaling. Disruption of complex II function is widely studied in the context of metabolic remodeling, hypoxia-associated signaling, and mitochondrial stress responses. Altered SDHA activity is also relevant to models of neurodegeneration and tumor biology where mitochondrial dysfunction and oncometabolite-like metabolic shifts are investigated.
SDHA Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Sdha locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Sdha. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Sdha function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Sdha-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.