Rtn-1A/B Antibody (RNL-3): sc-53008

Rtn-1A/B Antibody (RNL-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Rtn-1A/B protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Anti-Rtn-1A/B antibody (RNL-3) is available in both non-conjugated and various conjugated forms, including phycoerythrin (PE) and fluorescein isothiocyanate (FITC). The Reticulon (Rtn) family, which includes Rtn-1, Rtn-2, Rtn-3, and Nogo, plays a crucial role in the structure and function of the endoplasmic reticulum, with Rtn-1 being particularly significant due to its involvement in neuroendocrine secretion and membrane trafficking. The Rtn-1 gene, located on human chromosome 14q23.1, produces three isoforms—Rtn-1A, Rtn-1B, and Rtn-1C—through alternative splicing, and these isoforms are predominantly expressed in neural and neuroendocrine tissues. The structural characteristics of Rtn-1 proteins, including their common C-terminal regions that anchor them to the endoplasmic reticulum membranes, are vital for maintaining cellular integrity and facilitating intracellular transport processes. Notably, Rtn-1A has emerged as a reliable marker for neuroendocrine differentiation in lung cancer, as its expression is frequently observed in small cell lung carcinoma and carcinoid tumors, highlighting its potential as a diagnostic tool in oncology. Rtn-1B exists in multiple forms, while Rtn-1C expression is closely associated with neuronal differentiation, further underscoring the importance of these proteins in both normal physiology and disease states.

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Rtn-1A/B Antibody (RNL-3) References:

1. Cloning of a novel member of the reticulon gene family (RTN3): gene structure and chromosomal localization to 11q13.  |  Moreira, EF., et al. 1999. Genomics. 58: 73-81. PMID: 10331947
2. Identification of the Nogo inhibitor of axon regeneration as a Reticulon protein.  |  GrandPré, T., et al. 2000. Nature. 403: 439-44. PMID: 10667797
3. Subcellular localization and supramolecular organization of neuroendocrine-specific protein B (NSP-B) in small cell lung cancer.  |  Senden, NH., et al. 1994. Eur J Cell Biol. 65: 341-53. PMID: 7720728
4. NSP-encoded reticulons are neuroendocrine markers of a novel category in human lung cancer diagnosis.  |  van de Velde, HJ., et al. 1994. Cancer Res. 54: 4769-76. PMID: 8062278
5. Molecular cloning of a novel mouse gene with predominant muscle and neural expression.  |  Geisler, JG., et al. 1998. Mamm Genome. 9: 274-82. PMID: 9530622
6. Neuronal differentiation is accompanied by NSP-C expression.  |  Hens, J., et al. 1998. Cell Tissue Res. 292: 229-37. PMID: 9560466
7. cDNA cloning, genomic organization, and expression of the human RTN2 gene, a member of a gene family encoding reticulons.  |  Roebroek, AJ., et al. 1998. Genomics. 51: 98-106. PMID: 9693037

The preferred Rtn-1A antibody is Rtn-1A Antibody (Mon 160): sc-23880.