RFRP CRISPR/Cas9 KO Plasmid (h): sc-406767
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RFRP CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RFRP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RFRP CRISPR/Cas9 KO Plasmid (h) | sc-406767 | 20 µg | $397.00 |
Overview
NPVF encodes the RFamide-related peptide precursor that is processed to generate RFRP neuropeptides, which act as endogenous ligands for NPFF receptors and modulate neuroendocrine signaling. In humans, RFRP is implicated in hypothalamic control of gonadotropin release and reproductive axis timing, integrating cues that influence GnRH neuron activity and pituitary LH/FSH secretion. This pathway interfaces with broader GPCR-mediated neuromodulatory networks that affect stress responsiveness, energy balance, and circadian-linked reproductive physiology. Altered NPVF/RFRP signaling has been investigated in the context of reproductive disorders and dysregulated neuroendocrine states, supporting mechanistic studies of peptide processing, secretion, and receptor signaling.
RFRP CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NPVF gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NPVF together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NPVF open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RFRP protein expression.
This CRISPR knockout system enables efficient generation of NPVF-deficient cell models for investigation of RFRP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NPVF exon(s) critical for RFRP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NPVF genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RFRP CRISPR/Cas9 KO Plasmid (h) and RFRP CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NPVF locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RFRP HDR Plasmid (h) and RFRP HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NPVF homology arms to support homology-directed repair at defined NPVF target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.