RBQ-3 Antibody (F-7): sc-271072
- RBQ-3 Antibody (F-7) is a mouse monoclonal IgG2b κ RBQ-3 antibody provided at 200 µg/ml
- raised against amino acids 270-538 mapping at the C-terminus of RBQ-3 of human origin
- RBQ-3 Antibody (F-7) is recommended for detection of RBQ-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-RBQ-3 Antibody (F-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP is the preferred secondary detection reagent for RBQ-3 Antibody (F-7) for WB and IHC(P) applications. This reagent is now offered in a bundle with RBQ-3 Antibody (F-7) (see ordering information below).
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RBQ-3 Antibody (F-7) is a mouse monoclonal IgG2b kappa light chain antibody that detects RBQ-3 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). RBQ-3 (F-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RBQ-3, also known as retinoblastoma binding protein 5 (RBBP5) or SWD1, is a 538 amino acid protein that plays a crucial role in regulating cell proliferation by functioning as a component of the Set1 complex. Six WD repeats characterize RBQ-3, which are structural motifs facilitating protein-protein interactions essential for various cellular processes, including chromatin assembly and signal transduction. Nuclear localization of RBQ-3 enables interaction with key regulatory factors involved in cell cycle control and apoptosis. RBQ-3 preferentially binds to underphosphorylated forms of the retinoblastoma (Rb) protein, highlighting a potential role in tumor suppression. Upon DNA damage, ATM or ATR phosphorylates RBQ-3, which may influence cellular function and interactions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RBQ-3 Antibody (F-7) References:
- The WD repeat: a common architecture for diverse functions. | Smith, TF., et al. 1999. Trends Biochem Sci. 24: 181-5. PMID: 10322433
- WD-repeat proteins: structure characteristics, biological function, and their involvement in human diseases. | Li, D. and Roberts, R. 2001. Cell Mol Life Sci. 58: 2085-97. PMID: 11814058
- The WD-40 repeat. | van der Voorn, L. and Ploegh, HL. 1992. FEBS Lett. 307: 131-4. PMID: 1644165
- CUL4-DDB1 ubiquitin ligase interacts with multiple WD40-repeat proteins and regulates histone methylation. | Higa, LA., et al. 2006. Nat Cell Biol. 8: 1277-83. PMID: 17041588
- Identification and characterization of the human Set1B histone H3-Lys4 methyltransferase complex. | Lee, JH., et al. 2007. J Biol Chem. 282: 13419-28. PMID: 17355966
- Molecular cloning of a human protein that binds to the retinoblastoma protein and chromosomal mapping. | Saijo, M., et al. 1995. Genomics. 27: 511-9. PMID: 7558034
- The ancient regulatory-protein family of WD-repeat proteins. | Neer, EJ., et al. 1994. Nature. 371: 297-300. PMID: 8090199
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RBQ-3 Antibody (F-7) | sc-271072 | 200 µg/ml | $322.00 | |||
RBQ-3 Antibody (F-7): m-IgG2b BP-HRP Bundle | sc-548691 | 200 µg Ab; 10 µg BP | $361.00 | |||
RBQ-3 Antibody (F-7) AC | sc-271072 AC | 500 µg/ml, 25% agarose | $424.00 | |||
RBQ-3 Antibody (F-7) HRP | sc-271072 HRP | 200 µg/ml | $322.00 | |||
RBQ-3 Antibody (F-7) FITC | sc-271072 FITC | 200 µg/ml | $336.00 | |||
RBQ-3 Antibody (F-7) PE | sc-271072 PE | 200 µg/ml | $349.00 | |||
RBQ-3 Antibody (F-7) Alexa Fluor® 488 | sc-271072 AF488 | 200 µg/ml | $364.00 | |||
RBQ-3 Antibody (F-7) Alexa Fluor® 546 | sc-271072 AF546 | 200 µg/ml | $364.00 | |||
RBQ-3 Antibody (F-7) Alexa Fluor® 594 | sc-271072 AF594 | 200 µg/ml | $364.00 | |||
RBQ-3 Antibody (F-7) Alexa Fluor® 647 | sc-271072 AF647 | 200 µg/ml | $364.00 | |||
RBQ-3 Antibody (F-7) Alexa Fluor® 680 | sc-271072 AF680 | 200 µg/ml | $364.00 | |||
RBQ-3 Antibody (F-7) Alexa Fluor® 790 | sc-271072 AF790 | 200 µg/ml | $364.00 |