Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RBMX2 Double Nickase Plasmid (h): sc-412228-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RBMX2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RBMX2 Double Nickase Plasmid (h) and RBMX2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RBMX2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RBMX2 Antibody (C-1): sc-518215
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RBMX2 Double Nickase Plasmid (h) | sc-412228-NIC | 20 µg | $410.00 | |||
RBMX2 Double Nickase Plasmid (h2) | sc-412228-NIC-2 | 20 µg | $410.00 |
RBMX2 encodes an RNA-binding protein in the RBMX family that is implicated in post-transcriptional regulation, including pre-mRNA splicing and other RNA processing events that shape transcript isoform output. Through interactions with ribonucleoprotein complexes and spliceosomal machinery, RBMX2 is positioned to influence co- and post-transcriptional control of gene expression, impacting cell cycle progression, genome maintenance, and stress-responsive programs. Altered regulation of RNA processing pathways is a recurrent feature of malignant transformation and developmental disorders, making RBMX2 a useful target for dissecting how splicing-associated factors modulate cellular phenotypes. Human RBMX2 is therefore relevant for studies connecting RNA-binding proteins to transcription–splicing coupling and disease-associated transcriptome remodeling.
RBMX2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RBMX2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RBMX2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RBMX2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RBMX2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.