RBMX CRISPR/Cas9 KO Plasmid (h): sc-417339
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RBMX CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RBMX genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RBMX CRISPR/Cas9 KO Plasmid (h) | sc-417339 | 20 µg | $397.00 |
Overview
Human RBMX (RNA binding motif protein X-linked) is a nuclear RNA-binding protein that associates with nascent transcripts and contributes to pre-mRNA splicing, mRNA maturation, and the coordination of transcription with RNA processing. RBMX has been linked to genome maintenance through roles in the DNA damage response and replication-associated processes, supporting preservation of chromosomal integrity during cell cycle progression. Through its participation in spliceosome-related pathways and regulation of transcript isoform output, RBMX can influence gene expression programs governing proliferation and stress responses. Dysregulation of RBMX expression or function has been reported in cancer- and development-related contexts where altered RNA processing and genome stability are common pathogenic features.
RBMX CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RBMX gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RBMX together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RBMX open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RBMX protein expression.
This CRISPR knockout system enables efficient generation of RBMX-deficient cell models for investigation of RBMX signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RBMX exon(s) critical for RBMX function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RBMX genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RBMX CRISPR/Cas9 KO Plasmid (h) and RBMX CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RBMX locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RBMX HDR Plasmid (h) and RBMX HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RBMX homology arms to support homology-directed repair at defined RBMX target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.