RBM3 Double Nickase Plasmid (h): sc-406296-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RBM3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RBM3 Double Nickase Plasmid (h) and RBM3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RBM3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RBM3 Antibody (A-7): sc-390139
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RBM3 Double Nickase Plasmid (h) | sc-406296-NIC | 20 µg | $410.00 | |||
RBM3 Double Nickase Plasmid (h2) | sc-406296-NIC-2 | 20 µg | $410.00 |
RBM3 (RNA binding motif protein 3) is a cold-shock RNA-binding protein that modulates post-transcriptional gene regulation by influencing mRNA stability, splicing, and translation. It supports adaptive responses to cellular stress and has been linked to control of cell-cycle progression, apoptosis, and proteostasis through broad effects on RNA metabolism. Altered RBM3 expression has been associated with contexts of hypoxia, neuroprotection-related programs, and tumor biology, where it can correlate with changes in proliferation and stress tolerance. These properties make RBM3 a useful node for investigating RNA regulatory networks that shape cell fate decisions under physiological and stress conditions.
RBM3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RBM3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RBM3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RBM3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RBM3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.