Ran GAP1 Antibody (C-5): sc-28322

Ran GAP1 Antibody (C-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Ran GAP1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Ran GAP1 (C-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ran GAP1, also known as Ran GTPase Activating Protein 1, plays a crucial role in regulating the transport of proteins and RNA across the nuclear envelope, which is essential for maintaining cellular function and homeostasis. Located predominantly in the nucleus, Ran GAP1 is involved in the hydrolysis of GTP to GDP, thereby controlling the active and inactive states of the Ran GTPase. This regulation is vital for processes such as DNA replication and mitosis, as well as the transport of macromolecules through the nuclear pore complex. The interplay between Ran GAP1 and RCC1, which promotes the exchange of GDP for GTP, highlights the importance of Ran GAP1 in cellular signaling pathways. By modulating the levels of active Ran GTP, Ran GAP1 ensures that cellular processes are executed efficiently, making Ran GAP1 a key player in cell cycle regulation and nucleocytoplasmic transport.

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Ran GAP1 Antibody (C-5) References:

1. The Cellular Distribution of RanGAP1 Is Regulated by CRM1-Mediated Nuclear Export in Mammalian Cells.  |  Cha, K., et al. 2015. PLoS One. 10: e0141309. PMID: 26506250
2. Loss of RanGAP1 drives chromosome instability and rapid tumorigenesis of osteosarcoma.  |  Gong, Y., et al. 2023. Dev Cell. 58: 192-210.e11. PMID: 36696903
3. METTL3-mediated RanGAP1 promotes colorectal cancer progression through the MAPK pathway by recruiting YTHDF1.  |  Yang, R., et al. 2024. Cancer Gene Ther. 31: 562-573. PMID: 38267624
4. RanGAP1 maintains chromosome stability in limb bud mesenchymal cells during bone development.  |  Huang, M., et al. 2024. Cell Signal. 120: 111222. PMID: 38729327
5. GTP hydrolysis by Ran occurs at the nuclear pore complex in an early step of protein import.  |  Melchior, F., et al. 1995. J Cell Biol. 131: 571-81. PMID: 7593180
6. The Ran/TC4 GTPase-binding domain: identification by expression cloning and characterization of a conserved sequence motif.  |  Beddow, AL., et al. 1995. Proc Natl Acad Sci U S A. 92: 3328-32. PMID: 7724562
7. Protein export from the nucleus requires the GTPase Ran and GTP hydrolysis.  |  Moroianu, J. and Blobel, G. 1995. Proc Natl Acad Sci U S A. 92: 4318-22. PMID: 7753805
8. Interaction of the nuclear GTP-binding protein Ran with its regulatory proteins RCC1 and RanGAP1.  |  Klebe, C., et al. 1995. Biochemistry. 34: 639-47. PMID: 7819259
9. Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1.  |  Bischoff, FR., et al. 1995. EMBO J. 14: 705-15. PMID: 7882974
10. Crystal structure of the nuclear Ras-related protein Ran in its GDP-bound form.  |  Scheffzek, K., et al. 1995. Nature. 374: 378-81. PMID: 7885480
11. Separate domains of the Ran GTPase interact with different factors to regulate nuclear protein import and RNA processing.  |  Ren, M., et al. 1995. Mol Cell Biol. 15: 2117-24. PMID: 7891706
12. Catalysis of guanine nucleotide exchange of Ran by RCC1 and stimulation of hydrolysis of Ran-bound GTP by Ran-GAP1.  |  Bischoff, FR. and Ponstingl, H. 1995. Methods Enzymol. 257: 135-44. PMID: 8583915

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ran GAP1 Antibody (C-5) has 30 citations in a variety of scientific publications.

Primary antibodies like Anti-Ran GAP1 Antibody (C-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ran GAP1 Antibody (C-5) Specifications

Alternate Product Name: RANGAP1

Product Clone Name: C-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 408-587

Epitope Target: Ran GAP1

Epitope Species: human

Product Reactivity/Detection: Ran GAP1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 30 citations in various scientific publications

Available Conjugates/Forms for Ran GAP1 Antibody (C-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin