Rad1 Antibody (D-6): sc-166515

Rad1 Antibody (D-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects Rad1 protein in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rad1 Antibody (D-6) comes in non-conjugated form and various conjugates, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® options. Rad1 protein functions critically in DNA damage response, specifically regulating cell cycle checkpoints. Rad1 protein forms part of the 9-1-1 complex alongside Rad9 and Hus1, enabling Chk1 pathway activation during DNA damage response. This complex moves to DNA damage sites, facilitating repair and halting cell cycle progression until damage resolves. Proper Rad1 function maintains genomic stability, as disruption can lead to uncontrolled cell division and potential tumor development. Rad1 protein undergoes various post-translational modifications, including phosphorylation, affecting activity and protein interactions within DNA damage response pathways. Research on Rad1 protein interactions continues to advance cancer treatment strategies and expand understanding of cellular DNA damage responses.

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Rad1 Antibody (D-6) References:

1. HRad17, a human homologue of the Schizosaccharomyces pombe checkpoint gene rad17, is overexpressed in colon carcinoma.  |  Bao, S., et al. 1999. Cancer Res. 59: 2023-8. PMID: 10232579
2. Phosphorylation of Xenopus Rad1 and Hus1 defines a readout for ATR activation that is independent of Claspin and the Rad9 carboxy terminus.  |  Lupardus, PJ. and Cimprich, KA. 2006. Mol Biol Cell. 17: 1559-69. PMID: 16436514
3. The Rad9-Hus1-Rad1 (9-1-1) clamp activates checkpoint signaling via TopBP1.  |  Delacroix, S., et al. 2007. Genes Dev. 21: 1472-7. PMID: 17575048
4. Rad1, rad10 and rad52 mutations reduce the increase of microhomology length during radiation-induced microhomology-mediated illegitimate recombination in saccharomyces cerevisiae.  |  Chan, CY. and Schiestl, RH. 2009. Radiat Res. 172: 141-51. PMID: 19630519
5. Association of the Rad9-Rad1-Hus1 checkpoint clamp with MYH DNA glycosylase and DNA.  |  Hwang, BJ., et al. 2015. DNA Repair (Amst). 31: 80-90. PMID: 26021743
6. Excision repair of topoisomerase DNA-protein crosslinks (TOP-DPC).  |  Sun, Y., et al. 2020. DNA Repair (Amst). 89: 102837. PMID: 32200233
7. The chk1 pathway is required to prevent mitosis following cell-cycle arrest at 'start'.  |  Carr, AM., et al. 1995. Curr Biol. 5: 1179-90. PMID: 8548290
8. A human homolog of the Schizosaccharomyces pombe rad9+ checkpoint control gene.  |  Lieberman, HB., et al. 1996. Proc Natl Acad Sci U S A. 93: 13890-5. PMID: 8943031
9. Chk1 is a wee1 kinase in the G2 DNA damage checkpoint inhibiting cdc2 by Y15 phosphorylation.  |  O'Connell, MJ., et al. 1997. EMBO J. 16: 545-54. PMID: 9034337
10. Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25.  |  Sanchez, Y., et al. 1997. Science. 277: 1497-501. PMID: 9278511
11. Mitotic and G2 checkpoint control: regulation of 14-3-3 protein binding by phosphorylation of Cdc25C on serine-216.  |  Peng, CY., et al. 1997. Science. 277: 1501-5. PMID: 9278512
12. Hus1p, a conserved fission yeast checkpoint protein, interacts with Rad1p and is phosphorylated in response to DNA damage.  |  Kostrub, CF., et al. 1998. EMBO J. 17: 2055-66. PMID: 9524127

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rad1 Antibody (D-6) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Rad1 Antibody (D-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rad1 Antibody (D-6) Specifications

Alternate Product Name: Rad1

Product Clone Name: D-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-282

Epitope Target: Rad1

Epitope Species: human

Product Reactivity/Detection: Rad1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Rad1 Antibody (D-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Rad1 antibody is Rad1 Antibody (G-6): sc-166495.