PRH2 CRISPR/Cas9 KO Plasmid (h): sc-406292
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRH2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PRH2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRH2 CRISPR/Cas9 KO Plasmid (h) | sc-406292 | 20 µg | $397.00 |
Overview
PRH2 (proline-rich protein HaeIII subfamily 2) encodes a secreted, proline-rich salivary protein primarily expressed in salivary glands and released into the oral cavity. PRH2-derived proteins contribute to the composition and physicochemical properties of saliva, supporting lubrication, enamel pellicle formation, and interactions with dietary components and oral microbiota. Variation in salivary proline-rich proteins has been studied in the context of host–microbe interactions, oral ecology, and susceptibility to oral inflammatory conditions, where protein–surface binding and microbial adhesion can influence local immune and barrier processes. As a highly expressed secreted factor in a tissue-restricted context, PRH2 provides a useful model for dissecting secretory gene regulation and protein function in oral epithelial and glandular systems.
PRH2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PRH2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PRH2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PRH2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PRH2 protein expression.
This CRISPR knockout system enables efficient generation of PRH2-deficient cell models for investigation of PRH2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PRH2 exon(s) critical for PRH2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PRH2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PRH2 CRISPR/Cas9 KO Plasmid (h) and PRH2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PRH2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PRH2 HDR Plasmid (h) and PRH2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PRH2 homology arms to support homology-directed repair at defined PRH2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.