PMPCB CRISPR/Cas9 KO Plasmid (h): sc-405207
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PMPCB CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PMPCB genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PMPCB CRISPR/Cas9 KO Plasmid (h) | sc-405207 | 20 µg | $397.00 |
Overview
PMPCB encodes the mitochondrial processing peptidase beta subunit, a core component of the heterodimeric MPP complex that cleaves N-terminal targeting presequences from nuclear-encoded proteins after import into the mitochondrial matrix. By enabling maturation of oxidative phosphorylation and metabolic enzymes, PMPCB supports mitochondrial proteostasis, bioenergetic homeostasis, and stress responses linked to mitochondrial quality control. Disruption of mitochondrial presequence processing can impair respiratory chain function, elevate proteotoxic stress, and alter apoptosis and mitophagy signaling. PMPCB dysfunction and perturbed mitochondrial protein processing have been associated with neurodevelopmental phenotypes and mitochondrial disease mechanisms, making it relevant for studies of energy metabolism and organelle dysfunction.
PMPCB CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PMPCB gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PMPCB together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PMPCB open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PMPCB protein expression.
This CRISPR knockout system enables efficient generation of PMPCB-deficient cell models for investigation of PMPCB signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PMPCB exon(s) critical for PMPCB function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PMPCB genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PMPCB CRISPR/Cas9 KO Plasmid (h) and PMPCB CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PMPCB locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PMPCB HDR Plasmid (h) and PMPCB HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PMPCB homology arms to support homology-directed repair at defined PMPCB target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.