Molecular structure of Pladienolide B, CAS Number: 445493-23-2
Pladienolide B (CAS 445493-23-2)
See product citations (63)
Alternate Names:
Pla-B; (4R,7R,8S,9E,11S,12S)-8-(acetyloxy)-4,7-dihydroxy-12-[(1E,3E,5S)-6-[(2R,3R)-3-[(1R,2S)-2-hydroxy-1-methylbutyl]-2-oxiranyl]-1,5-dimethyl-1,3-oxacyclododec-9-en-2-one
Application:
Pladienolide B is a highly potent splicing inhibitor of both hypoxia signals and cancer cell proliferation
CAS Number:
445493-23-2
Molecular Weight:
536.70
Molecular Formula:
C30H48O8
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
Pladienolide B is a macrolide compound known for its role as a potent inhibitor of the spliceosome, a complex critical for the splicing of precursor messenger RNA in eukaryotic cells. It is of particular interest in molecular biology and genetics research, where it is used to study the mechanisms of mRNA splicing and to elucidate the consequences of spliceosome inhibition on gene expression. This compound has been employed to investigate the cellular processes that are impacted by altered splicing, which includes studies on cell cycle regulation and apoptosis. Through its action on the spliceosome, researchers have gained a better understanding of the complex interplay between splicing and various cellular pathways. The use of Pladienolide B in research helps to reveal the fundamental principles governing RNA biology and its implications in cellular function.
Pladienolide B (CAS 445493-23-2) References
- Targeting the spliceosome in chronic lymphocytic leukemia with the macrolides FD-895 and pladienolide-B. | Kashyap, MK., et al. 2015. Haematologica. 100: 945-54. PMID: 25862704
- Splicing function of mitotic regulators links R-loop-mediated DNA damage to tumor cell killing. | Wan, Y., et al. 2015. J Cell Biol. 209: 235-46. PMID: 25918225
- Splicing inhibition decreases phosphorylation level of Ser2 in Pol II CTD. | Koga, M., et al. 2015. Nucleic Acids Res. 43: 8258-67. PMID: 26202968
- Molecular identification of the dominant-negative, splicing isoform of the two-pore domain K(+) channel K(2P)5.1 in lymphoid cells and enhancement of its expression by splicing inhibition. | Endo, K., et al. 2015. Biochem Pharmacol. 98: 440-52. PMID: 26475531
- A Carbohydrate-Derived Splice Modulator. | Dhar, S., et al. 2016. J Am Chem Soc. 138: 5063-8. PMID: 27058259
- Selectivity in Small Molecule Splicing Modulation. | Kumar, D., et al. 2016. ACS Chem Biol. 11: 2716-2723. PMID: 27499047
- Pre-mRNA splicing repression triggers abiotic stress signaling in plants. | Ling, Y., et al. 2017. Plant J. 89: 291-309. PMID: 27664942
- Defective splicing of the background K+ channel K2P5.1 by the pre-mRNA splicing inhibitor, pladienolide B in lectin-activated mouse splenic CD4+ T cells. | Tagishi, K., et al. 2016. J Pharmacol Sci. 132: 205-209. PMID: 27865709
- Potential effect of spliceosome inhibition in small cell lung cancer irrespective of the MYC status. | Suda, K., et al. 2017. PLoS One. 12: e0172209. PMID: 28192473
- Pharmacological inhibition of the spliceosome subunit SF3b triggers exon junction complex-independent nonsense-mediated decay. | Carvalho, T., et al. 2017. J Cell Sci. 130: 1519-1531. PMID: 28302904
- Herboxidiene triggers splicing repression and abiotic stress responses in plants. | AlShareef, S., et al. 2017. BMC Genomics. 18: 260. PMID: 28347276
- SF3B1 is a stress-sensitive splicing factor that regulates both HSF1 concentration and activity. | Kim Guisbert, KS. and Guisbert, E. 2017. PLoS One. 12: e0176382. PMID: 28445500
- CRISPR/Cas9-mediated target validation of the splicing inhibitor Pladienolide B. | Aouida, M., et al. 2016. Biochim Open. 3: 72-75. PMID: 29450134
- Structural Basis of Splicing Modulation by Antitumor Macrolide Compounds. | Cretu, C., et al. 2018. Mol Cell. 70: 265-273.e8. PMID: 29656923
Inhibitor of:
2810011L19Rik, 2810403A07Rik, 82-FIP, 9130206N08Rik, AFF4, ANKRD13D, ARHGAP24, Arp7, ATXN1L, BAT1, Blood Group M antigen, BUD13, BUD31, BXDC5, C14orf38, C16orf33, C16orf57, C19orf29, C1orf55, C1orf63, C20orf78, C3orf14, C3orf38_4930453N24Rik, C6orf134, C7orf26, CACNG6, Caper, CASC3, CAT56, Cdc40, Cdc5L, CELF3, CELF6, CNOT10, CNOT8, CPSF3L, CPSF4, CPSF6, CPSF7, CRN, CROP, CstF-50, CstF-64, CTA, CWC15, CWC22, Dcp1, DDX17, DDX23, DDX25, DDX39, DDX40, DDX46, DDX51, DDX55, DPRX, DUX1, Edc3, Edc4, EF-CBP1, ELL2, Elongin A3, Esp31, EXOSC5, FAM165B, FUSIP1, GPATCH2, GPATCH3, GPATCH8, GRSF-1, GSE1, HEAB, HIV-1 Tat-SF1, hnRNP A/B, hnRNP A3, hnRNP C, hnRNP E1, hnRNP J, hnRNP L, hnRNP M, hnRNP Q, hnRNP U, IINTS1, INTS10, INTS3, INTS4, INTS8, INTS9, IQCF2, JK-1, KIAA0415, KIAA0649, KRR1, KRTAP4-5, LARP5, LARP7, LAS1L, Like-Sm (LSm), Liprin α4, LOC283143, LOC340094, LOC644838, LOC728392, LSm12, LSm4, LSm7, LUC7L2, MAGOH, Med2p, Mex3a, MFAP1A, MPP10, mrnp41, MRT4, NOL11, Nop10, Nop14, Nova-2, NPWBP, nRNP, NVL, NXF7, Obox1, OR52A5, OR8D2, p54/nrb, PABPN (poly(A) binding protein nuclear), PAP-α, PAP-γ, Pcf11, PHF3, PKDREJ, PLRG1, PNLDC1, PNRC1, POLR2E, POLR2I, Polr2j, POLR2J2, POLR2J3, PRKRIP1, PRP19, PRP4 kinase, PRP6, PRP8, PRPF18, PRPF3, PRPF31, PRPF38A, PRPF38B, PRPF39, PRPF4, PRPF40B, Rad21L1, RBM11, RBM12, RBM12B, RBM14, RBM15, RBM16, RBM17, RBM18, RBM20, RBM22, RBM23, RBM25, RBM29, RBM34, RBM35A, RBM4, RBM41, RBM7, RBM9, RBMS2, RBMXL1, RBMXL2, RBMY1, RBMY1E, RFXDC2, RNase K, RNPC3, RNPS1, ROD1, RP11-430L17.1, RP11-592B15.4, RPAIN, RPAP3, Rpb3, Rpp30, Rpp40, RSL24D1, Sab, SAP 14_SF3B14, SAP 145, SAP 155, SAP 49, SAP 61, SAP 62, SART-1, SC35, SCYL1BP1, SDCCAG10, SF2/ASF, SF3B10, SF3B3, SFRS11, SFRS12IP1, SFRS14, SFRS15, SFRS16, SFRS2B, SFRS2IP, SFRS8, Six6os1, Ski2, Sm B, Sm B′, Sm D2, Sm D3, Sm N, SmB, SMG5, SMNDC1, SMU1, SNRNP48, SNRPA, SON, splicing factor 1, SPT5, SRE-ZBP, SRp20, SRp30a, SRp30b, SRp30c, SRp75, SRRM4, SRrp130, SRrp35, Supt4h1, SYF2, TAF I p63_TAF I p68, TAF II p140, TAF7L, TAFII-18, TCEAL3, TCEAL5, TCEAL6, TFIIA-β, TFIIIC63, TFIIIC90, THOC1, TMEM98, Tra-2α, TRAP100, TRAP150, TSEN54, U11, U11/U12 snRNP 20K, U11/U12 snRNP 25, U2 snRNP A, U2 snRNP B, U2AF1L3, U2AF35, U2AF65, USP39, UTP15, VACVase, WBSCR27, WDR33, WDR43, WDR70, ZC3H12B, ZC3H14, ZCCHC11, ZCCHC2, ZCCHC4, ZCCHC7, ZCCHC8, ZFR2, ZFX, ZKSCAN4, ZNF131, ZNF16, ZNF19, ZNF258, ZNF265, ZNF425, ZNF500, ZNF563, ZNF579, ZNF593, ZNF662, ZNF671, ZNF696, ZNF7, ZNF707, ZNF768, ZNF852, ZNHIT2, ZRSR1, ZRSR2, and ZSCAN23.Activator of:
2810403A07Rik, 9G8, AQR, C16orf33, CBP80, CNOT1, DDX1, DDX28, DDX36, DIS3L, DOM3Z, DRIM, eIF4AIII, Gemin5, Gemin6, GPATCH4, hnRNP A/B, hnRNP A3, hnRNP H′/hnRNP H2, hnRNP H3, JKTBP, LSm11, MFAP1A, Nova-1, NTS12, PHF5A, PNO1, PNPase, Pol III RPC39, POLR3H, PRP2, PRPF (pre-mRNA processing factor), PTBP-2, RALYL, RBM32A, RBM4B, RBMXL1, RBMXL3, RBPMS2, Ribosomal Protein L26L1, RSRC2, SAP 155, SF4, SFRS12, SFRS17A, SLBP, SLU7, Sm E, Sm G, SNRPC, SR140, SRm160, SRp55, Srrp, TFIID, Topo IIIβ, TREX-2, U11/U12 snRNP 25, U2 snRNP B, U2 snRNP B′′, U2AF26, WDR4, and ZGPAT.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pladienolide B, 0.5 mg | sc-391691 | 0.5 mg | $299.00 | |||
Pladienolide B, 5 mg | sc-391691E | 5 mg | $2875.00 | |||
Pladienolide B, 10 mg | sc-391691B | 10 mg | $5699.00 | |||
Pladienolide B, 20 mg | sc-391691A | 20 mg | $11099.00 | |||
Pladienolide B, 50 mg | sc-391691C | 50 mg | $25500.00 | |||
Pladienolide B, 100 mg | sc-391691D | 100 mg | $66300.00 |