PIEZO1 Double Nickase Plasmid (h): sc-410555-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PIEZO1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PIEZO1 Double Nickase Plasmid (h) and PIEZO1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PIEZO1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PIEZO1 Double Nickase Plasmid (h) | sc-410555-NIC | 20 µg | $410.00 | |||
PIEZO1 Double Nickase Plasmid (h2) | sc-410555-NIC-2 | 20 µg | $410.00 |
PIEZO1 encodes a mechanosensitive, nonselective cation channel that converts membrane tension and shear stress into Ca²⁺ influx, enabling cells to sense and respond to mechanical cues. PIEZO1 activity regulates processes including cell volume control, ion homeostasis, cytoskeletal remodeling, and mechanotransduction pathways that influence migration, adhesion, and endothelial signaling. In hematologic and vascular contexts, PIEZO1-mediated Ca²⁺ signaling contributes to red blood cell hydration and deformability and to flow-dependent responses in endothelial cells. Genetic and functional perturbations of PIEZO1 have been linked to disorders of erythrocyte physiology and lymphatic/vascular development, making it a relevant target for dissecting mechano-regulated signaling networks.
PIEZO1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PIEZO1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PIEZO1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PIEZO1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PIEZO1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.