PICH CRISPR/Cas9 KO Plasmid (h): sc-407026
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PICH CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PICH genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PICH CRISPR/Cas9 KO Plasmid (h) | sc-407026 | 20 µg | $397.00 |
Overview
ERCC6L encodes PICH, a SNF2 family ATP-dependent DNA translocase that localizes to ultrafine DNA bridges during anaphase and promotes faithful chromosome segregation. PICH cooperates with DNA damage response and decatenation pathways by remodeling chromatin and coordinating the resolution of intertwined sister chromatids, supporting genome stability under replication stress. By regulating mitotic progression and preventing chromosome mis-segregation, PICH influences cellular outcomes linked to aneuploidy and genomic instability. Dysregulated ERCC6L/PICH activity has been associated with proliferative phenotypes and is studied in the context of cancer biology and other genome maintenance–related disorders.
PICH CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ERCC6L gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ERCC6L together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ERCC6L open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PICH protein expression.
This CRISPR knockout system enables efficient generation of ERCC6L-deficient cell models for investigation of PICH signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ERCC6L exon(s) critical for PICH function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ERCC6L genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PICH CRISPR/Cas9 KO Plasmid (h) and PICH CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ERCC6L locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PICH HDR Plasmid (h) and PICH HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ERCC6L homology arms to support homology-directed repair at defined ERCC6L target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.