PHF14 CRISPR/Cas9 KO Plasmid (h): sc-411313
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PHF14 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PHF14 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PHF14 CRISPR/Cas9 KO Plasmid (h) | sc-411313 | 20 µg | $397.00 |
Overview
PHF14 (PHD finger protein 14) encodes a chromatin-associated regulator implicated in transcriptional control through recognition of histone modifications and coordination of epigenetic states. It has been linked to regulation of cell differentiation programs, cell-cycle progression, and gene expression networks that shape cytoskeletal organization and developmental signaling. By modulating chromatin accessibility, PHF14 influences pathways involved in lineage specification and tissue homeostasis, with relevance to aberrant transcriptional regulation observed in cancer and developmental disorders. Research on PHF14 supports its use as a node for dissecting chromatin-driven phenotypes such as altered proliferation, migration, and context-dependent gene expression.
PHF14 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PHF14 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PHF14 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PHF14 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PHF14 protein expression.
This CRISPR knockout system enables efficient generation of PHF14-deficient cell models for investigation of PHF14 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PHF14 exon(s) critical for PHF14 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PHF14 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PHF14 CRISPR/Cas9 KO Plasmid (h) and PHF14 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PHF14 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PHF14 HDR Plasmid (h) and PHF14 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PHF14 homology arms to support homology-directed repair at defined PHF14 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.