Papilin CRISPR/Cas9 KO Plasmid (h): sc-406332
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Papilin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Papilin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Papilin CRISPR/Cas9 KO Plasmid (h) | sc-406332 | 20 µg | $397.00 |
Overview
PAPLN encodes papilin, a large extracellular matrix–associated glycoprotein characterized by multiple modular interaction domains that support matrix organization and basement membrane architecture. Papilin is implicated in extracellular protease regulation and matrix remodeling, processes that influence cell adhesion, migration, and tissue morphogenesis. Through these matrix-directed functions, PAPLN is relevant to pathways that couple extracellular matrix dynamics to developmental and pathological remodeling programs, including contexts where altered proteolysis and stromal organization contribute to disease biology. Studying PAPLN supports mechanistic work on extracellular matrix homeostasis and cell–matrix signaling interfaces.
Papilin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PAPLN gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PAPLN together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PAPLN open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Papilin protein expression.
This CRISPR knockout system enables efficient generation of PAPLN-deficient cell models for investigation of Papilin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PAPLN exon(s) critical for Papilin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PAPLN genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Papilin CRISPR/Cas9 KO Plasmid (h) and Papilin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PAPLN locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Papilin HDR Plasmid (h) and Papilin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PAPLN homology arms to support homology-directed repair at defined PAPLN target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.