PAOX CRISPR/Cas9 KO Plasmid (h): sc-406710
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PAOX CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PAOX genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PAOX CRISPR/Cas9 KO Plasmid (h) | sc-406710 | 20 µg | $397.00 |
Overview
Human PAOX encodes polyamine oxidase, a flavin-dependent enzyme that catalyzes the back-conversion of acetylated polyamines, contributing to cellular polyamine homeostasis and regulation of intracellular spermidine and spermine pools. PAOX activity links polyamine catabolism with redox biology through production of hydrogen peroxide and reactive aldehydes, influencing oxidative stress responses, mitochondrial function, and broader metabolic adaptation. Through these mechanisms, PAOX is studied in pathways governing cell proliferation, differentiation, and stress-induced signaling. Altered polyamine metabolism and PAOX-associated oxidative byproducts are frequently investigated in the context of cancer biology, neurodegeneration, and inflammatory processes as molecular correlates of dysregulated growth and cellular damage.
PAOX CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PAOX gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PAOX together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PAOX open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PAOX protein expression.
This CRISPR knockout system enables efficient generation of PAOX-deficient cell models for investigation of PAOX signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PAOX exon(s) critical for PAOX function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PAOX genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PAOX CRISPR/Cas9 KO Plasmid (h) and PAOX CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PAOX locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PAOX HDR Plasmid (h) and PAOX HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PAOX homology arms to support homology-directed repair at defined PAOX target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.