pan 14-3-3 Antibody (H-8): sc-1657

pan 14-3-3 Antibody (H-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects pan 14-3-3 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). pan 14-3-3 monoclonal antibody (H-8) is available in both conjugated and non-conjugated forms, providing versatility for various experimental needs. The 14-3-3 protein family plays a crucial role in regulating numerous cellular processes, particularly those related to cancer biology, such as apoptosis, mitogenic signaling, and cell-cycle checkpoints. This family consists of seven isoforms, including 14-3-3 β, γ, ε, ζ, η, θ, and σ, which form dimers that create two binding sites for ligand proteins, facilitating interactions that might not occur otherwise. 14-3-3 proteins are involved in the regulation of their ligand proteins through mechanisms such as cytoplasmic sequestration, modulation of interaction domains, and prevention of degradation. These proteins are known to interact with various target proteins, including kinases and phosphatases, which are essential for maintaining cellular homeostasis and signaling pathways. The ability of 14-3-3 proteins to bind to phosphorylated serine/threonine residues underscores their significance in cellular signaling, as alterations in their expression or function can lead to a wide range of pathological conditions, including cancer.

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pan 14-3-3 Antibody (H-8) References:

1. The 4.1/ezrin/radixin/moesin domain of the DAL-1/Protein 4.1B tumour suppressor interacts with 14-3-3 proteins.  |  Yu, T., et al. 2002. Biochem J. 365: 783-9. PMID: 11996670
2. MADM, a novel adaptor protein that mediates phosphorylation of the 14-3-3 binding site of myeloid leukemia factor 1.  |  Lim, R., et al. 2002. J Biol Chem. 277: 40997-1008. PMID: 12176995
3. The 14-3-3 cancer connection.  |  Hermeking, H. 2003. Nat Rev Cancer. 3: 931-43. PMID: 14737123
4. Isoform-specific subcellular localization among 14-3-3 proteins in Arabidopsis seems to be driven by client interactions.  |  Paul, AL., et al. 2005. Mol Biol Cell. 16: 1735-43. PMID: 15659648
5. 14-3-3 inhibition promotes dopaminergic neuron loss and 14-3-3θ overexpression promotes recovery in the MPTP mouse model of Parkinson's disease.  |  Ding, H., et al. 2015. Neuroscience. 307: 73-82. PMID: 26314634
6. 14-3-3 Proteins regulate mutant LRRK2 kinase activity and neurite shortening.  |  Lavalley, NJ., et al. 2016. Hum Mol Genet. 25: 109-22. PMID: 26546614
7. Dysregulation of 14-3-3 proteins in neurodegenerative diseases with Lewy body or Alzheimer pathology.  |  McFerrin, MB., et al. 2017. Ann Clin Transl Neurol. 4: 466-477. PMID: 28695147
8. Tau Aggregation-Dependent Lipid Peroxide Accumulation Driven by the hsa_circ_0001546/14-3-3/CAMK2D/Tau Complex Inhibits Epithelial Ovarian Cancer Peritoneal Metastasis.  |  Chai, B., et al. 2024. Adv Sci (Weinh). 11: e2310134. PMID: 38634567
9. 14-3-3: modulators of signaling proteins?  |  Morrison, D. 1994. Science. 266: 56-7. PMID: 7939645
10. Structural organization and chromosomal assignment of the human 14-3-3 eta chain gene (YWHAH).  |  Muratake, T., et al. 1996. Genomics. 36: 63-9. PMID: 8812417
11. The structural basis for 14-3-3:phosphopeptide binding specificity.  |  Yaffe, MB., et al. 1997. Cell. 91: 961-71. PMID: 9428519
12. A novel sphingosine-dependent protein kinase (SDK1) specifically phosphorylates certain isoforms of 14-3-3 protein.  |  Megidish, T., et al. 1998. J Biol Chem. 273: 21834-45. PMID: 9705322

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-pan 14-3-3 Antibody (H-8) has 239 citations in a variety of scientific publications.

Primary antibodies like Anti-pan 14-3-3 Antibody (H-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

pan 14-3-3 Antibody (H-8) Specifications

Alternate Product Name: 14-3-3

Product Clone Name: H-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-30

Epitope Target: 14-3-3 β

Epitope Species: human

Product Reactivity/Detection: pan 14-3-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 239 citations in various scientific publications

Available Conjugates/Forms for pan 14-3-3 Antibody (H-8): AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, TRITC: Rhodamine, X: TransCruz Reagent