Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
p47phox Double Nickase Plasmid (h): sc-417916-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- p47phox Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- p47phox Double Nickase Plasmid (h) and p47phox Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NCF1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: p47phox Antibody (D-10): sc-17845
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p47phox Double Nickase Plasmid (h) | sc-417916-NIC | 20 µg | $410.00 | |||
p47phox Double Nickase Plasmid (h2) | sc-417916-NIC-2 | 20 µg | $410.00 |
NCF1 encodes p47phox, a cytosolic organizer subunit of the phagocyte NADPH oxidase (NOX2) complex that regulates stimulus-dependent assembly of membrane and cytosolic components to generate reactive oxygen species (ROS). p47phox phosphorylation and membrane translocation help coordinate electron transfer from NADPH to oxygen, supporting oxidative burst responses, redox signaling, and microbial killing in myeloid cells. Altered NCF1 function perturbs ROS homeostasis and is linked to immunodeficiency phenotypes and inflammatory dysregulation, including susceptibility to chronic granulomatous disease–like presentations. In addition, NCF1 variation has been associated with autoimmune and autoinflammatory pathways, making p47phox a useful node for studying innate immune signaling and redox-dependent regulation.
p47phox Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NCF1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NCF1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NCF1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NCF1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.