p38 alpha MAPK14 Double Nickase Plasmid (h): sc-400057-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- p38 alpha MAPK14 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- p38 alpha MAPK14 Double Nickase Plasmid (h) and p38 alpha MAPK14 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MAPK14. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: p38 alpha MAPK14 Antibody (F-9): sc-271120
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p38 alpha MAPK14 Double Nickase Plasmid (h) | sc-400057-NIC | 20 µg | $410.00 | |||
p38 alpha MAPK14 Double Nickase Plasmid (h2) | sc-400057-NIC-2 | 20 µg | $410.00 |
MAPK14 encodes p38α, a stress-activated serine/threonine kinase in the MAPK family that integrates inflammatory cytokines, oxidative stress, and environmental cues. p38α regulates phosphorylation cascades controlling transcriptional programs, mRNA stability, and protein translation through pathways including MAPKAPK2/3, ATF2, and downstream modulation of NF-κB- and AP-1-linked responses. It influences apoptosis, cell-cycle checkpoints, differentiation, and innate immune signaling, with broad impact on cytokine production and cellular adaptation to stress. Dysregulated MAPK14 signaling has been implicated in inflammatory and autoimmune processes, neurodegeneration, and tumor-associated microenvironmental signaling, making it a frequent target for mechanistic pathway studies.
p38 alpha MAPK14 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MAPK14 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MAPK14. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MAPK14 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MAPK14-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.