Date published: 2025-9-8

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p35 Anticorpo (4G11): sc-293184

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Fichas de dados
  • p35 Anticorpo 4G11é um anticorpo monoclonal produzido em camundongo IgG2a κ p35 anticorpo, citado em 6 publicações, fornecido em 100 µg/ml
  • com anticorpos contra os aminoácidos 208-307 que representam um fragmento parcial de p35 human de origem
  • recomendado para a detecção de p35 de mouse, rat e human origem em
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de p35 (4G11): sc-293184.
  • Atualmente, ainda não concluímos a identificação do(s) reagente(s) de deteção secundário(s) preferido(s) para p35 Anticorpo (4G11). Este trabalho está em curso.

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O Anticorpo p35 (4G11) é um anticorpo monoclonal de cadeia leve IgG2a kappa de ratinho que detecta a proteína p35 de origem de camundongo, rato e humana por western blotting (WB), imunoprecipitação (IP), imunofluorescência (IF) e ensaio imunoenzimático (ELISA). O anticorpo anti-p35 (4G11) está disponível no formato não conjugado. A proteína p35, também conhecida como CDK5R1, desempenha um papel crucial na regulação da quinase dependente de ciclina 5 (Cdk5), que é essencial para a função e o desenvolvimento neuronais adequados. A Cdk5 é particularmente importante no cérebro, onde regula processos como a migração neuronal, a plasticidade sináptica e o controlo do ciclo celular. A interação da p35 com a Cdk5 é vital, uma vez que a p35 ativa a atividade cinase da Cdk5, permitindo que esta fosforile vários substratos envolvidos nestes processos neuronais críticos. O p35 é predominantemente expresso no cérebro, enquanto que uma forma truncada conhecida como p25 pode ser encontrada noutros tecidos, o que pode ter implicações em doenças neurodegenerativas quando o p25 se acumula. A especificidade e funcionalidade do anticorpo p35 (4G11) fazem do anticorpo p35 (4G11) uma ferramenta inestimável para os investigadores que estudam os papéis intrincados da Cdk5 e os mecanismos reguladores em estados normais e patológicos.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

p35 Anticorpo (4G11)

sc-293184
100 µg/ml
$316.00

培養細胞にてCDK5R1の発現についてWestern blottingでの解析,あるいは,剖検脳組織での局在をみるために免疫染色を計画しております.CDK5R1とp35の異同が分からず,混乱しております.NCBIのCDK5R1に関するサイトでは,「also known as」としてp35も記載されておりますが,同一と考えればよろしいのでしょうか?あるいは,どの抗体使用することが推奨されるでしょうか?

Asked by: himawari
質問頂き、誠にありがとうございます。CDK5R1はp35の別名である、この遺伝子によってコードされるタンパク質(p35)は、サイクリン依存性キナーゼ5(CDK5)のニューロン特異的活性化因子であり、CDK5の活性化は中枢神経系の適切な発達に必要である。https://www.uniprot.org/uniprotkb/Q15078/entry#names_and_taxonomy CDK5R1 is another name for p35.The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. https://www.uniprot.org/uniprotkb/Q15078/entry#names_and_taxonomy
Answered by: Jiajun Z
Date published: 2023-12-25

Hi! Does this antibody recognize p25, the proteolytic product of p35?

Asked by: Wayne Jiang
Thank you for your question. According to Uniprot, (http://www.uniprot.org/uniprot/Q15078#ptm_processing), the cleaved p25 form of p35 corresponds to amino acids 99-307. This mouse monoclonal antibody, p35 (4G11): sc-293184, is raised against amino acids 208-307 of human p35. Therefore, the antibody does have an epitope that should recognize the cleaved p25 form. We have not confirmed this experimentally.
Answered by: Technical Support
Date published: 2017-05-17

I am using p35 (4G11): sc-293184 for immunofluorescence, what dilution of this antibody should I use?

Asked by: jenniferc
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 2 out of 5 by from Not great at detecting endogenous proteinWorks at detecting overexpressed p35 on western blot pretty well, but not great when looking at endogenous p35- lots of other background bands. WB 1:1000 Overexpressed p35 in 293 cells or endogenous p35 from embryonic mouse brain (cortex) lysates.
Date published: 2017-11-18
Rated 3 out of 5 by from GoodOk, `36 kDa in 10% PAGE Used at an dilution of 1:250. Left lane loaded with protein standard marker 250, 130, 100, 55, 35, 25 kDa bands. Loaded with 35ug.
Date published: 2017-10-27
Rated 4 out of 5 by from works good for western blotwork well in WB at 1:1000,mouse.Strip is clear and the location is very accurate
Date published: 2017-10-08
Rated 4 out of 5 by from works good for western blotI got the free sample the last week and I got clear bands for western blot using lysates from cultured neurons and mouse brains. In addition, It successfully detects p35 and p25 in cell lysates. My PVDF membrane is probed with this antibody in 3% milk/TBST with 1:500 dilution at 4oC for 1 overnight.
Date published: 2017-09-19
Rated 4 out of 5 by from works greatlyI got the sample of this antibody and tested with breast cancer cell line with 1:1000. It works great with specific band at the right size.
Date published: 2017-08-20
Rated 4 out of 5 by from work for wbthe band is clear ,but the concentration is not high ,with 1:300
Date published: 2017-08-13
Rated 2 out of 5 by from good but need high concentrationI got the free sample and used for 15 ug of rat brain homogenate, with 1 to 200 dilution and enhanced ECL for 2 min we got the good signal.
Date published: 2017-08-10
Rated 1 out of 5 by from This p35 (4G11) antibody didn't work on WB.I got the p35 (4G11) antibody a few days ago and used the recommended secondary antibody sc-516102 to get the signal of LNCaP protein lysate by western blotting. There is nothing. No band (signal) on PVDF membrane. =>Primary antibody dilution is 1:1500~1:750, tried and got nothing. =>Plus secondary antibody dilution from 1:10000 to 1:5000, also got nothing. I got normal signal one the same target and same cell lysate and same WB method, the p35 (c-19) sc-820 works fine and perfect. Very sad!!
Date published: 2017-06-15
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p35 Anticorpo (4G11) is rated 3.0 out of 5 by 8.
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