p-Raf-1 Antibody (6B4): sc-81513
- p-Raf-1 Antibody (6B4) is a mouse monoclonal IgG2a, cited in 5 publications, provided at 50 µg/0.5 ml
- raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding serine 621 of Raf-1 of human origin
- recommended for detection of Ser 621 phosphorylated Raf-1 of mouse, rat and human origin by WB and IP
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-Raf-1 Antibody (6B4). This work is in progress.
p-Raf-1 Antibody (6B4) is a mouse monoclonal IgG2a antibody that detects p-Raf-1 in mouse, rat, and human samples through applications such as western blotting (WB) and immunoprecipitation (IP). p-Raf-1 is a critical regulator in the MAPK signaling pathway, primarily functioning as a serine/threonine kinase that plays a pivotal role in cell proliferation, differentiation, and survival. p-Raf-1 transduces signals from activated receptor tyrosine kinases, leading to the activation of downstream effectors like MEK and ERK, ultimately influencing cellular responses to growth factors and stress signals. p-Raf-1 undergoes several post-translational modifications, including phosphorylation at specific serine and tyrosine residues, which are essential for activation and function. Phosphorylation at Ser 338 is crucial for catalytic activity, while phosphorylation at Ser 621 is necessary for full activation. Phosphorylation of p-Raf-1 by protein kinase A (PKA) can inhibit activity, showcasing the intricate regulatory mechanisms that control function. anti-p-Raf-1 antibody (6B4) serves as an invaluable tool for researchers studying the dynamics of the Raf-1 signaling pathway and its implications in various physiological and pathological processes.
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p-Raf-1 Antibody (6B4) References:
- Serine and tyrosine phosphorylations cooperate in Raf-1, but not B-Raf activation. | Mason, CS., et al. 1999. EMBO J. 18: 2137-48. PMID: 10205168
- Interaction between active Pak1 and Raf-1 is necessary for phosphorylation and activation of Raf-1. | Zang, M., et al. 2002. J Biol Chem. 277: 4395-405. PMID: 11733498
- Phosphorylation of Raf-1 by p21-activated kinase 1 and Src regulates Raf-1 autoinhibition. | Tran, NH. and Frost, JA. 2003. J Biol Chem. 278: 11221-6. PMID: 12551923
- Baicalein inhibits Raf-1-mediated phosphorylation of MEK-1 in C6 rat glioma cells. | Nakahata, N., et al. 2003. Eur J Pharmacol. 461: 1-7. PMID: 12568909
- The role of Raf-1 phosphorylation in signal transduction. | Heidecker, G., et al. 1992. Adv Cancer Res. 58: 53-73. PMID: 1312290
- The RKIP (Raf-1 Kinase Inhibitor Protein) conserved pocket binds to the phosphorylated N-region of Raf-1 and inhibits the Raf-1-mediated activated phosphorylation of MEK. | Rath, O., et al. 2008. Cell Signal. 20: 935-41. PMID: 18294816
- Calcium/calmodulin-dependent protein kinase II (CaMKII) phosphorylates Raf-1 at serine 338 and mediates Ras-stimulated Raf-1 activation. | Salzano, M., et al. 2012. Cell Cycle. 11: 2100-6. PMID: 22592532
- Prognostic impact of Raf-1 and p-Raf-1 expressions for poor survival rate in non-small cell lung cancer. | Qiu, ZX., et al. 2012. Cancer Sci. 103: 1774-9. PMID: 22738312
- Clinical implications for loss or diminution of expression of Raf-1 kinase inhibitory protein and its phosphorylated form in ductal breast cancer. | Al-Mulla, F., et al. 2013. Am J Cancer Res. 3: 446-64. PMID: 24224123
- The Agonist of Adenosine A1 Receptor Induced Desensitization of delta Opioid receptor-mediated Raf-1/MEK/ERK Signaling by Feedback Phosphorylation of Raf-1-Ser289/296/301. | Xu, C., et al. 2023. Neurochem Res. 48: 1531-1542. PMID: 36525124
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-Raf-1 Antibody (6B4) | sc-81513 | 50 µg/0.5 ml | $322.00 |