p-HSP 27 Antibody (5B9): sc-81498

p-HSP 27 Antibody (5B9) is a mouse monoclonal IgG2a antibody that detects HSP 27 in mouse, rat, human, and canine samples through western blotting (WB) and immunoprecipitation (IP) applications. anti-HSP 27 antibody (5B9) specifically recognizes the phosphorylated form of HSP 27, which plays a crucial role in cellular stress responses and protects cells from damage during stressful conditions such as heat shock and oxidative stress. HSP 27 is predominantly located in the cytoplasm but translocates to the nucleus in response to stress, highlighting HSP 27′s importance in cellular signaling pathways that regulate survival and adaptation. Phosphorylation of HSP 27 at specific serine residues, particularly Ser 15, Ser 78, and Ser 82, is essential for function, as these modifications enhance chaperone activity and promote the formation of protein complexes that help in the refolding of denatured proteins. HSP 27′s ability to interact with various signaling molecules, including MAP kinase-activated protein kinase-2, underscores HSP 27′s significance in modulating cellular responses to stress and maintaining cellular homeostasis. With robust reactivity and specificity, HSP 27 monoclonal antibody (5B9) serves as an invaluable tool for researchers studying stress response mechanisms and heat shock protein roles in various diseases.

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p-HSP 27 Antibody (5B9) References:

1. TNF-alpha decreases hsp 27 in human blood mononuclear cells: involvement of protein kinase c.  |  Niwa, M., et al. 2006. Life Sci. 80: 181-6. PMID: 17069861
2. Vascular endothelial growth factor induces heat shock protein (HSP) 27 serine 82 phosphorylation and endothelial tubulogenesis via protein kinase D and independent of p38 kinase.  |  Evans, IM., et al. 2008. Cell Signal. 20: 1375-84. PMID: 18440775
3. Diminished phosphorylation of a heat shock protein (HSP 27) in infant acute lymphoblastic leukemia.  |  Strahler, JR., et al. 1991. Biochem Biophys Res Commun. 175: 134-42. PMID: 1998499
4. Hsp-27 induction requires POU4F2/Brn-3b TF in doxorubicin-treated breast cancer cells, whereas phosphorylation alters its cellular localisation following drug treatment.  |  Fujita, R., et al. 2011. Cell Stress Chaperones. 16: 427-39. PMID: 21279488
5. Heat shock proteins in the brain: role of Hsp70, Hsp 27, and HO-1 (Hsp32) and their therapeutic potential.  |  Sharp, FR., et al. 2013. Transl Stroke Res. 4: 685-92. PMID: 24323422
6. Palytoxin induces dissociation of HSP 27 oligomers through a p38 protein kinase pathway.  |  Berni, C., et al. 2015. Chem Res Toxicol. 28: 752-64. PMID: 25710824
7. Hsp-27 and NF-κB pathway is associated with AR/AR-V7 expression in prostate cancer cells.  |  Kiliccioglu, I., et al. 2019. Gene. 697: 138-143. PMID: 30807779
8. Geranylgeranylacetone attenuates cerebral ischemia-reperfusion injury in rats through the augmentation of HSP 27 phosphorylation: a preliminary study.  |  Matsuo, K., et al. 2021. BMC Neurosci. 22: 9. PMID: 33557752
9. Interleukin (IL)-6 signaling leads to phosphorylation of the small heat shock protein (Hsp)27 through activation of the MAP kinase and MAPKAP kinase 2 pathway in monocytes and monocytic leukemia cells.  |  Belka, C., et al. 1995. Leukemia. 9: 288-94. PMID: 7869766
10. A role for the p38 mitogen-activated protein kinase/Hsp 27 pathway in cholecystokinin-induced changes in the actin cytoskeleton in rat pancreatic acini.  |  Schäfer, C., et al. 1998. J Biol Chem. 273: 24173-80. PMID: 9727040

The preferred HSP 27 antibody is HSP 27 Antibody (F-4): sc-13132.