p-CKR-5 Antibody (V14/2): sc-32303

p-CKR-5 Antibody (V14/2) is a mouse monoclonal IgG2b antibody that detects CKR-5 in human samples through western blotting (WB) and immunoprecipitation (IP) applications. CKR-5, a member of the C-C chemokine receptor family, plays a crucial role in the immune response by acting as a receptor for various chemokines, which are essential for the chemotaxis of immune cells such as T cells and monocytes to sites of inflammation. Notably, CKR-5 serves as a coreceptor for HIV, facilitating viral entry into host cells, particularly those that are macrophage-tropic. CKR-5′s structure includes a seven-pass transmembrane domain, which is vital for signal transduction. Upon binding of an agonist, CKR-5 undergoes phosphorylation at four distinct serine residues located in its carboxy terminus, a modification that is critical for interaction with G-proteins and subsequent receptor desensitization. This phosphorylation not only regulates CKR-5′s activity but also influences CKR-5′s ability to mediate immune responses and viral infection, making CKR-5 a significant target for therapeutic interventions in HIV and inflammatory diseases.

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p-CKR-5 Antibody (V14/2) References:

1. Differential effects of CC chemokines on CC chemokine receptor 5 (CCR5) phosphorylation and identification of phosphorylation sites on the CCR5 carboxyl terminus.  |  Oppermann, M., et al. 1999. J Biol Chem. 274: 8875-85. PMID: 10085131
2. A new insight into the role of 'old' chemotactic peptide receptors FPR and FPRL1: down-regulation of chemokine receptors CCR5 and CXCR4.  |  Le, Y., et al. 1999. Forum (Genova). 9: 299-314. PMID: 10611407
3. Down-regulation of the chemokine receptor CCR5 by activation of chemotactic formyl peptide receptor in human monocytes.  |  Shen, W., et al. 2000. Blood. 96: 2887-94. PMID: 11023526
4. Pharmacological characterization of the chemokine receptor, CCR5.  |  Mueller, A., et al. 2002. Br J Pharmacol. 135: 1033-43. PMID: 11861332
5. Analysis of ligand-stimulated CC chemokine receptor 5 (CCR5) phosphorylation in intact cells using phosphosite-specific antibodies.  |  Pollok-Kopp, B., et al. 2003. J Biol Chem. 278: 2190-8. PMID: 12403770
6. CCL3, acting via the chemokine receptor CCR5, leads to independent activation of Janus kinase 2 (JAK2) and Gi proteins.  |  Mueller, A. and Strange, PG. 2004. FEBS Lett. 570: 126-32. PMID: 15251452
7. Vaccinia virus activation of CCR5 invokes tyrosine phosphorylation signaling events that support virus replication.  |  Rahbar, R., et al. 2006. J Virol. 80: 7245-59. PMID: 16809330
8. Chemokine co-receptor CCR5/CXCR4-dependent modulation of Kv2.1 channel confers acute neuroprotection to HIV-1 glycoprotein gp120 exposure.  |  Shepherd, AJ., et al. 2013. PLoS One. 8: e76698. PMID: 24086760
9. The Abrogation of Phosphorylation Plays a Relevant Role in the CCR5 Signalosome Formation with Natural Antibodies to CCR5.  |  Venuti, A., et al. 2017. Viruses. 10: PMID: 29283386
10. Importance of PLC-Dependent PI3K/AKT and AMPK Signaling in RANTES/CCR5 Mediated Macrophage Chemotaxis.  |  Chien, HC., et al. 2018. Chin J Physiol. 61: 266-279. PMID: 30384400

The preferred CKR-5 antibody is CKR-5 Antibody (D-6): sc-17833.