OGFR CRISPR/Cas9 KO Plasmid (h): sc-407303
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OGFR CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the OGFR genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OGFR CRISPR/Cas9 KO Plasmid (h) | sc-407303 | 20 µg | $397.00 |
Overview
Opioid growth factor receptor (OGFR) is a ubiquitously expressed, non-classical opioid receptor that binds [Met5]-enkephalin (opioid growth factor) to regulate cell proliferation and tissue homeostasis. OGFR signaling is best characterized through the OGF–OGFR axis, which modulates cell-cycle progression by influencing cyclin-dependent kinase inhibitor pathways and constraining DNA synthesis in diverse cell types. This receptor has been implicated in epithelial renewal, immune and stromal cell behavior, and stress-responsive growth control, linking OGFR biology to contexts such as cancer cell proliferation, wound repair, and inflammatory pathology. As a result, perturbation of OGFR expression is commonly used to dissect endogenous growth-inhibitory signaling networks and their crosstalk with mitogenic pathways.
OGFR CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the OGFR gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the OGFR together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the OGFR open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish OGFR protein expression.
This CRISPR knockout system enables efficient generation of OGFR-deficient cell models for investigation of OGFR signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting OGFR exon(s) critical for OGFR function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple OGFR genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by OGFR CRISPR/Cas9 KO Plasmid (h) and OGFR CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the OGFR locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by OGFR HDR Plasmid (h) and OGFR HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by OGFR homology arms to support homology-directed repair at defined OGFR target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.