Oct-2 Antibody (F-5): sc-377475
- Oct-2 Antibody (F-5) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 435-463 at the C-terminus of Oct-2 of human origin
- recommended for detection of Oct-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine
- TransCruz reagent for ChIP application (sc-377475 X, 200 µg/0.1 ml)
- See Oct-2 (PT2): sc-56822 for Oct-2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Oct-2 Antibody (F-5) for WB and IHC(P) applications. These reagents are now offered in bundles with Oct-2 Antibody (F-5) (see ordering information below).
Oct-2 Antibody (F-5) is a mouse monoclonal IgG1 antibody that detects Oct-2 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Oct-2 plays a crucial role in the regulation of gene expression, particularly in the immune system, where Oct-2 is essential for the activation of immunoglobulin gene transcription in B cells. Oct-2 is primarily located in the nucleus, where Oct-2 binds to specific DNA sequences known as octamer motifs, which are vital for the transcription of immunoglobulin genes. Nuclear localization of Oct-2 allows for precise regulation of gene expression in response to cellular signals, ensuring that B cells can produce antibodies effectively during immune responses. Additionally, Oct-2 interacts with other transcription factors and coactivators, such as Oct-1 and OBF1, to form a complex that enhances the transcriptional activity of immunoglobulin promoters, thereby facilitating the production of antibodies necessary for adaptive immunity. Anti-Oct-2 antibody (F-5) is an invaluable tool for researchers studying B cell biology and the mechanisms of immune regulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Oct-2 Antibody (F-5) References:
- Analysis of transcription factor OCT.1, OCT.2 and BOB.1 expression using tissue arrays in classical Hodgkin's lymphoma. | García-Cosío, M., et al. 2004. Mod Pathol. 17: 1531-8. PMID: 15257313
- Differential transcriptional activation by Oct-1 and Oct-2: interdependent activation domains induce Oct-2 phosphorylation. | Tanaka, M. and Herr, W. 1990. Cell. 60: 375-86. PMID: 2302733
- Oct-2 forms a complex with Oct-1 on the iNOS promoter and represses transcription by interfering with recruitment of RNA PolII by Oct-1. | Bentrari, F., et al. 2015. Nucleic Acids Res. 43: 9757-65. PMID: 26271992
- Fampridine is a Substrate and Inhibitor of Human OCT2, but not of Human MATE1, or MATE2K. | Xiao, G., et al. 2018. Pharm Res. 35: 159. PMID: 29915999
- Targeting OCT2 with Duloxetine to Prevent Oxaliplatin-Induced Peripheral Neurotoxicity. | Nepal, MR., et al. 2022. Cancer Res Commun. 2: 1334-1343. PMID: 36506732
- Characterization of ligand-induced thermal stability of the human organic cation transporter 2 (OCT2). | Maane, M., et al. 2023. Front Pharmacol. 14: 1154213. PMID: 37007010
- Molecular basis of polyspecific drug and xenobiotic recognition by OCT1 and OCT2. | Suo, Y., et al. 2023. Nat Struct Mol Biol. 30: 1001-1011. PMID: 37291422
- Effect of 7-ketocholesterol incorporation on substrate binding affinity and turnover rate of the organic cation transporter 2 (OCT2). | Xiu, F., et al. 2024. Biochem Pharmacol. 220: 116017. PMID: 38176620
- Mouse Oct-1 contains a composite homeodomain of human Oct-1 and Oct-2. | Suzuki, N., et al. 1993. Nucleic Acids Res. 21: 245-52. PMID: 8441632
- Molecular principles of Oct2-mediated gene activation in B cells. | Wirth, T., et al. 1995. Immunobiology. 193: 161-70. PMID: 8530140
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Oct-2 Antibody (F-5) | sc-377475 | 200 µg/ml | $322.00 | |||
Oct-2 Antibody (F-5): m-IgG Fc BP-HRP Bundle | sc-540726 | 200 µg Ab; 10 µg BP | $361.00 | |||
Oct-2 Antibody (F-5): m-IgG1 BP-HRP Bundle | sc-542327 | 200 µg Ab; 20 µg BP | $361.00 | |||
Oct-2 (F-5) Neutralizing Peptide | sc-377475 P | 100 µg/0.5 ml | $69.00 | |||
Oct-2 Antibody (F-5) X | sc-377475 X | 200 µg/0.1 ml | $322.00 |