Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Notch 4 CRISPR/Cas9 KO Plasmid (m): sc-421934
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Notch 4 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Notch 4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Notch 4 Antibody (A-12): sc-393893
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Notch 4 CRISPR/Cas9 KO Plasmid (m) | sc-421934 | 20 µg | $397.00 |
Overview
Notch4 encodes a single-pass transmembrane receptor in the Notch family that mediates contact-dependent signaling controlling cell fate decisions, proliferation, and differentiation. Upon ligand engagement by Delta-like or Jagged proteins, receptor proteolysis releases the Notch intracellular domain to regulate transcriptional programs in coordination with RBPJ/CSL and co-activators. In mouse tissues, Notch4 is strongly linked to vascular and endothelial biology, influencing angiogenic behavior and vessel maturation through crosstalk with pathways such as VEGF, Wnt, and TGF-β. Dysregulated Notch4 signaling has been associated with aberrant vascular remodeling and tumor-associated angiogenesis, making it relevant for mechanistic studies of developmental and disease-associated signaling networks.
Notch 4 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Notch4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Notch4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Notch4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Notch 4 protein expression.
This CRISPR knockout system enables efficient generation of Notch4-deficient cell models for investigation of Notch 4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Notch4 exon(s) critical for Notch 4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Notch4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Notch 4 CRISPR/Cas9 KO Plasmid (m) and Notch 4 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Notch4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Notch 4 HDR Plasmid (m) and Notch 4 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Notch4 homology arms to support homology-directed repair at defined Notch4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.