Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Nopp140 Double Nickase Plasmid (h): sc-402907-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nopp140 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Nopp140 Double Nickase Plasmid (h) and Nopp140 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NOLC1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nopp140 Antibody (E-7): sc-374033
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nopp140 Double Nickase Plasmid (h) | sc-402907-NIC | 20 µg | $410.00 | |||
Nopp140 Double Nickase Plasmid (h2) | sc-402907-NIC-2 | 20 µg | $410.00 |
NOLC1 encodes nucleolar and coiled-body phosphoprotein 1 (Nopp140), a highly phosphorylated nucleolar protein that shuttles between the nucleolus and Cajal bodies to support ribosome biogenesis and small nucleolar RNP/small Cajal body–specific RNP dynamics. Nopp140 interacts with core components of rRNA transcription and processing machinery and helps organize nucleolar architecture, linking its function to RNA polymerase I activity, pre-rRNA maturation, and nucleolar stress responses. Perturbation of NOLC1 can alter rRNA processing, nucleolar integrity, and cell-cycle progression, making it relevant for mechanistic studies of growth control and stress signaling. Because nucleolar dysfunction is a common feature of diverse pathophysiological states, NOLC1 is frequently examined as a marker and regulator of nucleolar homeostasis in disease-relevant cellular models.
Nopp140 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NOLC1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NOLC1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NOLC1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NOLC1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.